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Titolo:
CONSTITUTIVE EXPRESSION OF CHIMERIC NEO-REV RESPONSE ELEMENT TRANSCRIPTS SUPPRESSES HIV-1 REPLICATION IN HUMAN CD4(-LYMPHOCYTES() T)
Autore:
BEVEC D; VOLCPLATZER B; ZIMMERMANN K; DOBROVNIK M; HAUBER J; VERES G; BOHNLEIN E;
Indirizzi:
PROGENESYS,3400 W BAYSHORE RD PALO ALTO CA 94303 PROGENESYS PALO ALTO CA 94303 SANDOZ GMBH,RES INST,ART DEPT VIENNA AUSTRIA UNIV VIENNA,INST VIROL,LSGT,VIRCC A-1095 VIENNA AUSTRIA
Titolo Testata:
Human gene therapy
fascicolo: 2, volume: 5, anno: 1994,
pagine: 193 - 201
SICI:
1043-0342(1994)5:2<193:CEOCNR>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; TYPE-1 REPLICATION; TRANS-ACTIVATOR; CELLS; GENE; SEQUENCE; INVITRO; OVEREXPRESSION; INHIBITION; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
D. Bevec et al., "CONSTITUTIVE EXPRESSION OF CHIMERIC NEO-REV RESPONSE ELEMENT TRANSCRIPTS SUPPRESSES HIV-1 REPLICATION IN HUMAN CD4(-LYMPHOCYTES() T)", Human gene therapy, 5(2), 1994, pp. 193-201

Abstract

We have previously reported that chimeric neomycin phosphotransferase(neo)-Rev response element (RRE) transcripts suppress the function ofthe human immunodeficiency virus type 1 (HIV-1) Rev trans-activator protein in HeLa cells. In an extension of these experiments, human CD4() CEM cells (G418-resistant cell populations and clonal isolates) stably expressing chimeric neo-RRE genes (2, 3, or 6 RRE copies) were generated using retroviral-mediated gene transfer. The transduced CEM clones were infected with the HIV-1 HTLV(IIIb) isolate and the following three phenotypes were observed: (i) the transduced CEM cells were readily infected with HIV-1 indistinguishable from the control CEM cells; (ii) the appearance of HIV-1 replication markers was significantly delayed; (iii) no signs of HIV-1 replication were detectable although proviral HIV-1 DNA sequences could be detected in these cells. Furthermore, HIV antigen expression was limited in neo-resistant CEM cell populations inoculated with the HIV-1 HTLV(IIIb) isolate. Only 10% of the CEM-pX17-3xRRE cells and 20% of the CEM-pX17-2xRRE cells displayed HIV-1antigens 43 days after challenge and had retained CD4 surface expression on 47% and 64% of the cells, respectively. In sharp contrast, 80% of the CEM-pX17 or the CEM-pX17-6xRRE cells expressed HIV-1 antigens but no CD4 antigens were detectable in these cultures. These results clearly indicate that RRE decoys could be developed into an effective somatic gene therapy approach against HIV-1 induced acquired immunodeficiency syndrome (AIDS).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 03:09:40