Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ROLES OF ANTIBODIES AND COMPLEMENT IN PHAGOCYTIC KILLING OF ENTEROCOCCI
Autore:
ARDUINO RC; MURRAY BE; RAKITA RM;
Indirizzi:
UNIV TEXAS,HSC,CTR INFECT DIS,6431 FANNIN,ROOM 1728 JFB HOUSTON TX 77030 UNIV TEXAS,SCH MED,CTR INFECT DIS,DEPT INTERNAL MED HOUSTON TX 77030 UNIV TEXAS,SCH MED,DEPT MICROBIOL & MED GENET HOUSTON TX 77030
Titolo Testata:
Infection and immunity
fascicolo: 3, volume: 62, anno: 1994,
pagine: 987 - 993
SICI:
0019-9567(1994)62:3<987:ROAACI>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
STREPTOCOCCUS-FAECALIS; BETA-LACTAMASE; SEX-PHEROMONES; RESISTANCE; ENDOCARDITIS; PLASMIDS; INVITRO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
R.C. Arduino et al., "ROLES OF ANTIBODIES AND COMPLEMENT IN PHAGOCYTIC KILLING OF ENTEROCOCCI", Infection and immunity, 62(3), 1994, pp. 987-993

Abstract

The contributions of complement and antibodies to polymorphonuclear leukocyte (PMN)-mediated killing of enterococci were investigated with pooled normal human serum (PNHS) or immune human sera (IHS) from patients with serious enterococcal infections. Each IHS containing antienterococcal antibodies demonstrated by enzyme-linked immunosorbent assay and Western blotting (immunoblotting) was examined with the enterococcus strain isolated from the same patient. PNHS promoted PMN-mediated kilting of enterococci similar to that for IHS. PMN-mediated killing was consistently abrogated after preopsonization with heat-inactivated PNHS, but some heat-inactivated IHS supported neutrophil bactericidal activity. Inhibition of the classical pathway of complement by chelation of either PNHS or IHS with Mg-EGTA [Mg-ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid] did not alter PMN-mediated killing, suggesting that activation of the alternative pathway of complement is sufficient to promote killing of enterococci by PMNs. PMN-mediated killing assays were also performed with normal rabbit serum and immune rabbit serum against enterococci. Preopsonization with heat-inactivated immune rabbit serum resulted in PMN-mediated killing of enterococci; which was ablatedd after adsorption of the serum with the sameisolate used for immunization. The influence of different phenotypic enterococcal traits on neutrophil-mediated killing was also investigated. Similar kinetics of killing were observed for derivatives of Enterocccus faecalis strains regardless of resistance to antimicrobial agents or production of p-lactamase, hemolysin, gelatinase, or surface proteins involved in the aggregative response to pheromones. In summary, PMN-mediated killing of enterococci appears to depend primarily on complement activation by either the classical or the alternative pathway. Human antienterococcal antibodies generated during infection variablypromoted neutrophil bactericidal activity, while antibody raised in arabbit supported PMN-mediated killing of the organism examined. Finally, the different phenotypic properties of E. faecalis examined did not influence the neutrophil-mediated killing of these organisms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 09:05:42