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Titolo:
EXPRESSION OF CMS-UNIQUE AND FLANKING MITOCHONDRIAL-DNA SEQUENCES IN PHASEOLUS-VULGARIS L
Autore:
CHASE CD;
Indirizzi:
UNIV FLORIDA,INST FOOD & AGR SCI,DEPT HORT SCI GAINESVILLE FL 32611
Titolo Testata:
Current genetics
fascicolo: 3, volume: 25, anno: 1994,
pagine: 245 - 251
SICI:
0172-8083(1994)25:3<245:EOCAFM>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOPLASMIC MALE-STERILITY; PLANT-MITOCHONDRIA; MESSENGER-RNA; OENOTHERA MITOCHONDRIA; FERTILITY RESTORATION; YEAST MITOCHONDRIA; INTRON SEQUENCES; SPLICING INVITRO; GENE; TRANSCRIPTION;
Keywords:
MITOCHONDRIA; TRANSCRIPTION; POLYRIBOSOMES; FERTILITY RESTORATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
C.D. Chase, "EXPRESSION OF CMS-UNIQUE AND FLANKING MITOCHONDRIAL-DNA SEQUENCES IN PHASEOLUS-VULGARIS L", Current genetics, 25(3), 1994, pp. 245-251

Abstract

The expression of mitochondrial DNA sequences unique to a cytoplasmically male-sterile (CMS) line of Phaseolus vulgaris was investigated. RNA-blot hybridizations with strand-specific probes demonstrated CMS-unique transcripts (7.0, 6.8, 4.7, 3.3 and 2.8 kb) to be in the sense orientation with respect to the longest open reading frames within the CMS-unique region. Hybridizations revealed co-transcription of CMS-unique and upstream, atpA-coding sequences to generate the 6.8-kb RNA. However, hybridizations with CMS-unique and flanking DNA probes accountedfor only 4.9 kb of the longest and most abundant (7.0 kb) CMS-unique transcript, providing indirect evidence for the involvement of a splicing process in the generation of this transcript. Sedimentation experiments demonstrated the association of 7.0- and 6.8-kb CMS-unique transcripts with polyribosomes in seedlings and floral buds of a CMS line and a line restored to fertility by the nuclear gene Fr2. However, steady-state levels of the 7.0- and 6.8-kb transcripts were decreased in the restored line relative to the CMS line.

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Documento generato il 05/12/20 alle ore 00:16:56