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Titolo:
DISTINCT UPSTREAM ACTIVATION REGIONS FOR GLUCOSE-REPRESSED AND DEREPRESSED EXPRESSION OF THE YEAST CITRATE SYNTHASE GENE CIT1
Autore:
ROSENKRANTZ M; KELL CS; PENNELL EA; WEBSTER M; DEVENISH LJ;
Indirizzi:
VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT MICROBIOL IMMUNOL RICHMOND VA 23298
Titolo Testata:
Current genetics
fascicolo: 3, volume: 25, anno: 1994,
pagine: 185 - 195
SICI:
0172-8083(1994)25:3<185:DUARFG>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION INITIATION SITES; CYTOCHROME-C GENE; SACCHAROMYCES-CEREVISIAE; LACZ FUSIONS; S-CEREVISIAE; CYC1 GENE; MITOCHONDRIAL; DEHYDROGENASE; PROTEINS; DNA;
Keywords:
YEAST; CITRATE SYNTHASE; TRANSCRIPTIONAL REGULATION; HAP2,3,4;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
63
Recensione:
Indirizzi per estratti:
Citazione:
M. Rosenkrantz et al., "DISTINCT UPSTREAM ACTIVATION REGIONS FOR GLUCOSE-REPRESSED AND DEREPRESSED EXPRESSION OF THE YEAST CITRATE SYNTHASE GENE CIT1", Current genetics, 25(3), 1994, pp. 185-195

Abstract

The yeast CIT1 (mitochondrial citrate synthase) gene is subject to glucose repression and is further repressed by glucose plus glutamate. Based on deletion analysis of a CIT1-lacZ gene fusion, DNA sequences between -548 and -273 are required for full expression of CIT1. The region of transcription initiation and the putative TATA element are located at -150 to -100 and -195 respectively. A restriction fragment containing DNA sequences between -457 and -211 conferred activation and glucose-glutamate regulation when placed in either orientation upstream of a UAS-less heterologous yeast gene. Deletion of DNA sequences between -291 and -273 specifically eliminated derepression of CIT1, and destroyed one of two closely-spaced, potential binding sites for the HAP2,3,4 transcriptional activator protein. Ten-base-pair block substitutions in the region -367 to -348 reduced glucose-repressed expression. Thus, it appears that distinct DNA sequences upstream of CIT1 activate expression in glucose-repressed and derepressed cells. Possible mechanisms of regulation by glutamate plus glucose, are discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 21:47:05