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Titolo:
HUMANIZATION OF MOUSE ANTI-HUMAN IL-2 RECEPTOR ANTIBODY B-B10
Autore:
NAKATANI T; LONE YC; YAMAKAWA J; KANAOKA M; GOMI H; WIJDENES J; NOGUCHI H;
Indirizzi:
SUMITOMO PHARMACEUT CO LTD,DISCOVERY RES LABS 2,RES CTR,KONOHANA KU,1-98 KASUGADE NAKA 3 OSAKA 554 JAPAN SUMITOMO CHEM CO LTD,TAKARAZUKA RES CTR,BIOTECHNOL LAB TAKARAZUKA HYOGO 665 JAPAN CTR REG TRANSFUS SANGUINE F-25020 BESANCON FRANCE
Titolo Testata:
Protein engineering
fascicolo: 3, volume: 7, anno: 1994,
pagine: 435 - 443
SICI:
0269-2139(1994)7:3<435:HOMAIR>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESHAPING HUMAN-ANTIBODIES; HUMAN MONOCLONAL-ANTIBODY; HYPERVARIABLE REGIONS; HUMAN-IMMUNOGLOBULIN; IMMUNE-RESPONSE; THERAPY; EXPRESSION; GENES; RESOLUTION; SEGMENTS;
Keywords:
CDR GRAFT; COMPUTER MODELING; HUMANIZED ANTIBODY; SITE-DIRECTED MUTAGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
T. Nakatani et al., "HUMANIZATION OF MOUSE ANTI-HUMAN IL-2 RECEPTOR ANTIBODY B-B10", Protein engineering, 7(3), 1994, pp. 435-443

Abstract

Mouse monoclonal anti-human IL-2 receptor antibody (B-B10) inhibits IL-2-dependent human T-cell proliferation. It has been used in clinicaltrials in the transplantation field and promising results are being accumulated. Mouse B-B10 antibody was humanized by grafting all CDRs and some framework amino acid residues onto human antibodies, KAS for V-H and PAY for V-k. Nine humanized B-B10s with differently grafted framework residues were constructed and assessed for their biological activities. One of these humanized B-B10, M5, showed nearly the same activity as the mouse B-B10. The 49th residue of V-k was demonstrated to play a crucial role in the antigen-antibody interaction by 3-D structureanalysis with a computer modeling system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 23:43:18