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Titolo:
MODULATION OF FUSICOCCIN-BINDING PROTEIN-ACTIVITY IN MUNG BEAN (VIGNA-RADIATA L) HYPOCOTYLS BY TISSUE MATURATION AND BY FUSICOCCIN
Autore:
BASEL LE; ZUKOWSKI AT; CLELAND RE;
Indirizzi:
UNIV WASHINGTON,DEPT BOT SEATTLE WA 98195 UNIV WASHINGTON,DEPT BOT SEATTLE WA 98195
Titolo Testata:
Plant physiology
fascicolo: 2, volume: 104, anno: 1994,
pagine: 691 - 697
SICI:
0032-0889(1994)104:2<691:MOFPIM>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEMBRANE H+-ATPASE; PLASMA-MEMBRANE; CORN COLEOPTILES; HIGHER-PLANTS; PURIFICATION; RECEPTORS; TOXIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
L.E. Basel et al., "MODULATION OF FUSICOCCIN-BINDING PROTEIN-ACTIVITY IN MUNG BEAN (VIGNA-RADIATA L) HYPOCOTYLS BY TISSUE MATURATION AND BY FUSICOCCIN", Plant physiology, 104(2), 1994, pp. 691-697

Abstract

The phytotoxin fusicoccin (FC), after binding to a plasma membrane-localized receptor, causes higher plant cells to excrete protons. Ligand-binding analysis has been used to show that the plasma membrane of mung bean (Vigna radiata L.) hypocotyls contains both high-affinity (HA)and tow-affinity (LA) binding sites for FC. The effect of tissue maturation on these sites was determined on isolated membrane vesicles from the meristematic region (hook) and the elongation zone and from mature hypocotyl tissues. In the meristematic region the HA:LA ratio was 1:20. As hypocotyl tissues matured, the site density of HA increased and there was no change in LA density, so that the HA:LA ratio increasedto 1:2 in mature tissues. PC-induced proton excretion correlates withthe HA density, not the LA density. When sections isolated from each region were incubated with FC prior to isolation of membranes, there was an apparent conversion of LA to HA sites during the first 90 min inall regions. During the next 1 to 3 h there was a further 2.5- to 3-fold increase in binding sites in all regions, accompanied by a slight decline in dissociation constant. The increase in binding sites, but not the apparent conversion of LA to HA, was partly blocked by cycloheximide. These data suggest that FC alters FC-binding protein activity in two ways: first, by causing an increase in affinity for FC of preexisting LA receptors, and second by inducing the synthesis of additionalFC receptors. This apparent up-regulation of a phytotoxin receptor byits ligand in plants has not previously been reported.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 09:56:52