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Titolo:
A HISTONE-BINDING PROTEIN, NUCLEOPLASMIN, STIMULATES TRANSCRIPTION FACTOR-BINDING TO NUCLEOSOMES AND FACTOR-INDUCED NUCLEOSOME DISASSEMBLY
Autore:
CHEN H; LI BY; WORKMAN JL;
Indirizzi:
PENN STATE UNIV,DEPT BIOCHEM & MOLEC BIOL,301 ALTHOUSE LAB UNIV PK PA16802 PENN STATE UNIV,DEPT BIOCHEM & MOLEC BIOL UNIV PK PA 16802 PENN STATE UNIV,CTR GENE REGULAT UNIV PK PA 16802
Titolo Testata:
EMBO journal
fascicolo: 2, volume: 13, anno: 1994,
pagine: 380 - 390
SICI:
0261-4189(1994)13:2<380:AHPNST>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
XENOPUS-LAEVIS OOCYTES; TUMOR VIRUS PROMOTER; DNA-REPLICATION INVITRO; CHROMATIN STRUCTURE; HYPERSENSITIVE SITES; ASSEMBLY INVITRO; MAMMALIAN-CELLS; PURIFICATION; COMPLEXES; NUCLEI;
Keywords:
HISTONES; NUCLEOPLASMIN; NUCLEOSOME; TRANSCRIPTION FACTOR; TRANSCRIPTION REGULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
64
Recensione:
Indirizzi per estratti:
Citazione:
H. Chen et al., "A HISTONE-BINDING PROTEIN, NUCLEOPLASMIN, STIMULATES TRANSCRIPTION FACTOR-BINDING TO NUCLEOSOMES AND FACTOR-INDUCED NUCLEOSOME DISASSEMBLY", EMBO journal, 13(2), 1994, pp. 380-390

Abstract

The binding of a GAL4-AH, USF or Sp1 to nucleosome cores was stimulated by the presence of the histone-binding protein, nucleoplasmin. Stimulation of factor binding by nucleoplasmin was specific for nucleosomereconstituted DNA and was not mimicked by non-specific histone sinks (i.e. polyglutamate or RNA). Upon GAL4-AH binding, nucleoplasmin specifically removed histones H2A and H2B from the nucleosome which enhanced the subsequent loss of the H3/H4 tetramers onto competing DNA. Thus,nucleoplasmin participated in the complete conversion of nucleosome cores to transcription factor - DNA complexes. These data indicate thatproteins which bind histones can increase transcription factor binding to nucleosomal DNA and that transcription factor binding can initiate nucleosome disassembly. Similar activities of histone-binding proteins may participate in the displacement of nucleosomes at enhancers andpromoters in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 10:48:31