Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
TRANSCRIPTIONAL REPRESSION BY NUCLEOSOMES BUT NOT H1 IN RECONSTITUTEDPREBLASTODERM DROSOPHILA CHROMATIN
Autore:
SANDALTZOPOULOS R; BLANK T; BECKER PB;
Indirizzi:
EUROPEAN MOLEC BIOL LAB,GENE EXPRESS PROGRAMME D-69012 HEIDELBERG GERMANY EUROPEAN MOLEC BIOL LAB,GENE EXPRESS PROGRAMME D-69012 HEIDELBERG GERMANY
Titolo Testata:
EMBO journal
fascicolo: 2, volume: 13, anno: 1994,
pagine: 373 - 379
SICI:
0261-4189(1994)13:2<373:TRBNBN>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA POLYMERASE-II; MINICHROMOSOMES ASSEMBLED INVITRO; HEAT-SHOCK GENES; HISTONE-H1; DNA; ACTIVATION; EMBRYOS; MELANOGASTER; INITIATION; EXPRESSION;
Keywords:
CHROMATIN STRUCTURE; HISTONE H1; NUCLEOSOME; PREBLASTODERM EMBRYOS; REPRESSION OF TRANSCRIPTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
R. Sandaltzopoulos et al., "TRANSCRIPTIONAL REPRESSION BY NUCLEOSOMES BUT NOT H1 IN RECONSTITUTEDPREBLASTODERM DROSOPHILA CHROMATIN", EMBO journal, 13(2), 1994, pp. 373-379

Abstract

Chromatin reconstituted in an extract from preblastoderm Drosophila embryos represses transcription by RNA polymerase II. We have assembledregularly spaced nucleosomes on DNA attached to paramagnetic beads enabling the efficient purification of chromatin templates for transcription studies. We have used diagnostic salt extractions to establish that transcriptional repression of immobilized chromatin was largely dueto nucleosome cores. When purified H1 was incorporated into chromatin, resulting in increased repeat lengths to 200-220 bp, the contribution of H1 to transcriptional repression was negligible. If more H1 was added no regularly spaced chromatin was obtained and only under these conditions was transcriptional inhibition by H1 apparent. We conclude that efficient repression of transcription by polymerase II in this system does not require the presence of histone H1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 16:16:21