Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
COMPARISON OF SUPEROVULATORY RESPONSE OF MATURE OUTBRED MICE TREATED WITH FSH OR PMSG AND DEVELOPMENTAL POTENTIAL OF EMBRYOS PRODUCED
Autore:
MUNOZ I; DESADIA CR; GUTIERREZ A; BLANQUEZ MJ; PINTADO B;
Indirizzi:
INIA,CIT,DEPT PROD ANIM,CTRA LA CORUNA KM 5900 E-28040 MADRID SPAIN INIA,CIT,DEPT PROD ANIM E-28040 MADRID SPAIN
Titolo Testata:
Theriogenology
fascicolo: 4, volume: 41, anno: 1994,
pagine: 907 - 914
SICI:
0093-691X(1994)41:4<907:COSROM>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
FOLLICLE-STIMULATING-HORMONE; CONTINUOUS INFUSION; MOUSE; CLEAVAGE; RATS;
Keywords:
SUPEROVULATION; MICE; FSH; PMSG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
I. Munoz et al., "COMPARISON OF SUPEROVULATORY RESPONSE OF MATURE OUTBRED MICE TREATED WITH FSH OR PMSG AND DEVELOPMENTAL POTENTIAL OF EMBRYOS PRODUCED", Theriogenology, 41(4), 1994, pp. 907-914

Abstract

A superovulatory treatment for mice based on FSH administration was compared with a standard one based on PMSG. Our aim was to determine ifa mean number of embryos recovered per donor could be increased and if in vitro or in vivo viability was affected by the hormonal treatmentused. Thus, female Swiss mice were subjected to 2 superovulatory treatments, and the 1-cell and 2-cell stage embryos were cultured in 2 different media to the blastocyst stage or were transferred to pseudopregnant recipients. The data show that despite a lower mating percentage (52% with FSH vs 66% with PMSG), the FSH-treated mice provided twice the number of total embryos (53.4 vs 24.5) with a similar percentage ofmorphologically normal embryos (74% for FSK vs 69% for PMSG). We alsofound that in vitro culture results can be influenced by the source of gonadotropins depending on the culture medium used. A culture mediumsuch as CZB which prevents the 2-cell block, provided the same developmental rates regardless of hormonal treatment used. However, with M-16 medium, which does not prevent this blockage, only 39% of the 2-cellFSH-derived embryos and 49% of the PMSG-derived 2-cell embryos developed into blastocysts (P<0.05). FSH-derived embryos resulted in a higher percentage of pregnant recipients (73 vs 56%) than PMSG-derived embryos, but the number of alive fetuses and the number of implantations per pregnant recipient was affected only by the kind of culture system used before transfer. The results dow that FSH can provide very good superovulatory response in mice, thus reducing the number of donors needed for a given experiment and providing embryos of at least the same quality as those derived from the standard PMSG treatment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:38:22