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Titolo:
LOCALIZATION OF OXYTOCIN RECEPTOR MESSENGER-RNA IN THE OVINE UTERUS DURING THE ESTROUS-CYCLE AND EARLY-PREGNANCY
Autore:
STEVENSON KR; RILEY PR; STEWART HJ; FLINT APF; WATHES DC;
Indirizzi:
AFRC,BABRAHAM INST,DEPT CELLULAR PHYSIOL CAMBRIDGE CB2 4AT ENGLAND AFRC,BABRAHAM INST,DEPT CELLULAR PHYSIOL CAMBRIDGE CB2 4AT ENGLAND INST ZOOL LONDON NW1 4RY ENGLAND UNIV NOTTINGHAM,DEPT PHYSIOL & ENVIRONM SCI LOUGHBOROUGH LE12 5RD LEICS ENGLAND
Titolo Testata:
Journal of molecular endocrinology
fascicolo: 1, volume: 12, anno: 1994,
pagine: 93 - 105
SICI:
0952-5041(1994)12:1<93:LOORMI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONCEPTUS SECRETORY PROTEINS; ESTROUS-CYCLE; TROPHOBLAST PROTEIN-1; PLASMA-CONCENTRATIONS; OVARIECTOMIZED EWES; UTERINE SECRETION; PROSTAGLANDIN-F; ENDOMETRIUM; RELEASE; SHEEP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
58
Recensione:
Indirizzi per estratti:
Citazione:
K.R. Stevenson et al., "LOCALIZATION OF OXYTOCIN RECEPTOR MESSENGER-RNA IN THE OVINE UTERUS DURING THE ESTROUS-CYCLE AND EARLY-PREGNANCY", Journal of molecular endocrinology, 12(1), 1994, pp. 93-105

Abstract

A synthetic 45-mer oligonucleotide corresponding to part of the ovineendometrial oxytocin receptor cDNA was hybridized to sections of ovine uterus collected from 40 ewes at different stages during the oestrous cycle, the first 3 weeks of pregnancy and seasonal anoestrus. The quantity of oxytocin receptor mRNA was measured as the optical density (OD) value on autoradiographs using image analysis. Message first appeared in the luminal epithelium on days 14-15 of the cycle, increasing to a peak OD of 0.48 at oestrus and decreasing again between days 2 and5. Oxytocin receptor mRNA in the superficial glands, deep glands and caruncular stroma increased between day 15 and oestrus to peak OD values of 0.17, 0.11 and 0.11 respectively, declining again by day 2 and reaching basal values (OD<0.015) by day 5. Hybridization to the myometrium tended to rise from a mean OD value of 0.01 on days 2-15 to a peakof 0.03 +/- 0.01 (mean +/- S.E.M.) on days 0-1, but the change was not significant. In pregnant ewes there was no detectable oxytocin receptor mRNA on days 14-15 in any region, but hybridization to the luminalepithelium was present in two of three ewes on day 21. In anoestrous ewes oxytocin receptor mRNA concentrations in all areas of the endometrium were approximately half those measured at oestrus. Optical density readings for oxytocin receptor mRNA in the various uterine compartments were compared with measurements of oxytocin receptors in the same regions as assessed by binding studies using the I-125-labelled oxytocin antagonist d(CH2)(5)[Tyr(Me)(2),Thr(4),Tyr-NH29]-vasotocin (I-125-labelled OTA). In the endometrium, receptor mRNA and I-125-labelled OTAbinding patterns changed in parallel, and both sets of measurements were significantly correlated (P<0.01). In the myometrium, a significant increase in I-125-labelled OTA binding occurred at oestrus; this wasnot accompanied by a similar increase in oxytocin receptor mRNA hybridization. This study helps to confirm that the previously identified cDNA clone is derived from the ovine oxytocin receptor, as patterns of oxytocin receptor mRNA expression in the endometrium closely resembledthose of oxytocin binding. Maximum expression and binding both occurred at oestrus, suggesting that regulation of the oxytocin receptor gene in the uterus occurs principally at the transcriptional, rather thanat the translational, level. Failure to detect a significant increasein myometrial mRNA expression at oestrus may indicate that the endometrial and myometrial oxytocin receptors are of different isoforms.

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Documento generato il 24/09/20 alle ore 03:27:15