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Titolo:
CYTOPLASMIC ARGYROPHILIC PROTEIN(S) IN CUMULUS-OOCYTE COMPLEXES - A PRELIMINARY-STUDY
Autore:
ANTALIKOVA L; FULKA J; HORSKA M; PETR J;
Indirizzi:
RES INST ANIM PROD CS-10400 PRAGUE 10 CZECH REPUBLIC RES INST ANIM PROD CS-10400 PRAGUE 10 CZECH REPUBLIC
Titolo Testata:
Reproduction, nutrition, development
fascicolo: 1, volume: 34, anno: 1994,
pagine: 73 - 80
SICI:
0926-5287(1994)34:1<73:CAPICC>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEIOTIC RESUMPTION; MATURATION; CYCLOHEXIMIDE; SEQUENCE; INVITRO;
Keywords:
CUMULUS OOPHORUS; OOCYTE MATURATION; FARM MAMMALS; ARGYROPHILIC PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
L. Antalikova et al., "CYTOPLASMIC ARGYROPHILIC PROTEIN(S) IN CUMULUS-OOCYTE COMPLEXES - A PRELIMINARY-STUDY", Reproduction, nutrition, development, 34(1), 1994, pp. 73-80

Abstract

The presence of acidic argyrophilic protein(s) in cumulus cells and oocytes was examined in bovine, ovine, porcine and murine cumulus-oocyte complexes (COCs) using a specific silver-staining method. The COCs were isolated from small (1 mm) or large (3-5 mm) antral follicles; they were fixed immediately after slaughter (first group) or after transfer to the laboratory (ca 60 min, second group) and then silver stainedaccording to Likovsky' and Smetana (1981). The argyrophilic proteins were accumulated mainly in foot processes of cumulus cells on oocytes isolated from large bovine follicles. The intensity of the reaction was less evident in small follicles. The intensity of staining graduallydisappeared from cumulus cells as the maturation continued. Moreover,we were unable to detect similar strong labelling in mouse, pig and sheep COCs. Such a chronology suggests that argyrophilic protein(s) mayplay a role in the transition from meiotic arrest to resumption in bovine oocytes. Our results also suggest that preliminary events of maturation may occur just after slaughter, ie a relatively long time before the COCs are isolated and cultured in vitro.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 10:17:12