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Titolo:
INHIBITION OF FERTILITY IN FEMALE MICE BY IMMUNIZATION WITH A B-CELL EPITOPE, THE SYNTHETIC SPERM PEPTIDE, P10G
Autore:
ORAND MG; BEAVERS J; WIDGREN EE; TUNG KSK;
Indirizzi:
UNIV N CAROLINA,DEPT CELL BIOL & ANAT,210A TAYLOR HALL,CB 7090 CHAPELHILL NC 27599 UNIV VIRGINIA,DEPT PATHOL CHARLOTTESVILLE VA 22903
Titolo Testata:
Journal of reproductive immunology
fascicolo: 2, volume: 25, anno: 1993,
pagine: 89 - 102
SICI:
0165-0378(1993)25:2<89:IOFIFM>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN; INFERTILITY; SPERMATOZOA;
Keywords:
SPERM ANTIGEN; CONTRACEPTIVE VACCINE; SPERM PEPTIDE; B-CELL EPITOPE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
M.G. Orand et al., "INHIBITION OF FERTILITY IN FEMALE MICE BY IMMUNIZATION WITH A B-CELL EPITOPE, THE SYNTHETIC SPERM PEPTIDE, P10G", Journal of reproductive immunology, 25(2), 1993, pp. 89-102

Abstract

Human patient sera containing antisperm antibodies (from vasectomizedmen and infertile women) immunologically react with the synthetic peptide P10G (PGGGTLPPSG), and affinity purified antibodies to P10G (anti-P10G) react with human spermatozoa (O'Rand et al., 1990). In this study P10G was used to elicit antibodies and the effect of the antibodieson fertility in female mice determined. The P10G sequence is derived from the 14-kDa rabbit sperm autoantigen, RSA (O'Rand and Widgren, 1990). The results of this study demonstrate that female mice can become infertile when immunized with the synthetic peptide P10G conjugated tothe carrier protein keyhole limpet hemocyanin (KLH). However, the results also show that it is important to distinguish those mice with high serum antibody levels from those with lower levels. Infertility was clearly apparent in the high titer subgroup with an 80% decrease in pregnancy rate during the last of three matings and a 71% decrease in litter size over all three matings when compared to the low titer subgroup or the control groups. Significantly, mice immunized with P10G without carrier protein show no detectable antigen specific proliferation of lymph node cells in response to 100 mu M of the peptide. P10G is not a T-cell epitope, but rather a B-cell epitope and it does not elicitan autoimmune response in the female mouse. This demonstration in mice is an important first step in the development of a safe human immunocontraceptive vaccine.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 18:13:03