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Titolo:
A MONOCLONAL-ANTIBODY THAT BLOCKS POLIOVIRUS ATTACHMENT RECOGNIZES THE LYMPHOCYTE HOMING RECEPTOR CD44
Autore:
SHEPLEY MP; RACANIELLO VR;
Indirizzi:
COLUMBIA UNIV,COLL PHYSICIANS & SURGEONS,DEPT MICROBIOL,701 W 168TH ST NEW YORK NY 10032 HARVARD UNIV,SCH MED,DEPT PATHOL BOSTON MA 02115 DANA FARBER CANC INST BOSTON MA 02115
Titolo Testata:
Journal of virology
fascicolo: 3, volume: 68, anno: 1994,
pagine: 1301 - 1308
SICI:
0022-538X(1994)68:3<1301:AMTBPA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-CELL ACTIVATION; HUMAN-ERYTHROCYTE ANTIGENS; SURFACE GLYCOPROTEINS; MOLECULAR-CLONING; HIGH ENDOTHELIUM; IN(LU) GENE; HUMAN PGP-1; HELA-CELLS; EXPRESSION; ADHESION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
M.P. Shepley e V.R. Racaniello, "A MONOCLONAL-ANTIBODY THAT BLOCKS POLIOVIRUS ATTACHMENT RECOGNIZES THE LYMPHOCYTE HOMING RECEPTOR CD44", Journal of virology, 68(3), 1994, pp. 1301-1308

Abstract

A monoclonal antibody, AF3, was previously shown to specifically inhibit poliovirus binding to HeLa cells and to detect a 100-kDa glycoprotein only in cell lines and tissues permissive for poliovirus infection. These results suggested that the 100-kDa protein may be involved in the pathogenesis of poliomyelitis and the cellular function of the poliovirus receptor site. To study further the role of the 100-kDa protein in poliovirus attachment, immunoaffinity purification, amino acid sequencing, and cDNA cloning were undertaken. The results demonstrate that antibody AF3 reacts with the lymphocyte homing receptor CD44, a multifunctional cell surface glycoprotein involved in the homing of circulating lymphocytes to lymph nodes and the modulation of lymphocyte adhesion and activation. Antibody AF3 reacts with a subset of CD44 molecules ((AF3)CD44H), which appears to be a small fraction of the heterogeneously glycosylated CD44 molecules expressed on hematopoietic and nonhematopoietic cells. Anti-CD44 monoclonal antibodies, previously reported to induce CD44-mediated modulation of lymphocyte activation and adhesion, compete with I-125-AF3 in binding assays, demonstrating functional overlap among the epitopes. The anti-CD44 monoclonal antibody A3D8, which binds to a greater molecular weight range of CD44 than does AF3, inhibits poliovirus binding to a similar degree. CD44 does not actas a poliovirus receptor, since CD44-expressing mouse L-cell transformants did not bind poliovirus. The poliovirus receptor and (AF3)CD44H may be noncovalently associated, or they may interact through the cytoskeleton or signal transduction pathways.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 07:14:19