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Titolo:
DIFFERENTIAL TISSUE EXPRESSION OF IMMUNOREACTIVE DEHYDROPEPTIDASE-I, A PEPTIDYL LEUKOTRIENE METABOLIZING ENZYME
Autore:
INAMURA T; PARDRIDGE WM; KUMAGAI Y; BLACK KL;
Indirizzi:
UNIV CALIF LOS ANGELES,MED CTR,BRAIN RES INST,DEPT MED,DIV NEUROSURG,74-140 CHS LOS ANGELES CA 90024 UNIV CALIF LOS ANGELES,MED CTR,BRAIN RES INST,DEPT MED,DIV NEUROSURG LOS ANGELES CA 90024 UNIV CALIF LOS ANGELES,MED CTR,DEPT PHARMACOL LOS ANGELES CA 90024
Titolo Testata:
Prostaglandins, leukotrienes and essential fatty acids
fascicolo: 2, volume: 50, anno: 1994,
pagine: 85 - 92
SICI:
0952-3278(1994)50:2<85:DTEOID>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD-TUMOR BARRIER; RENAL DIPEPTIDASE; INTRACAROTID INFUSION; AFFINITY PURIFICATION; BRAIN-BARRIER; PERMEABILITY; LOCALIZATION; THIENAMYCIN; GLUTATHIONE; MEDIATORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
T. Inamura et al., "DIFFERENTIAL TISSUE EXPRESSION OF IMMUNOREACTIVE DEHYDROPEPTIDASE-I, A PEPTIDYL LEUKOTRIENE METABOLIZING ENZYME", Prostaglandins, leukotrienes and essential fatty acids, 50(2), 1994, pp. 85-92

Abstract

We previously reported that intracarotid infusion of leukotriene C-4 (LTC(4)) causes a selective increase in vascular permeability within brain tumor capillaries in experimental rat brain tumor. Normal brain capillaries are rich in gamma-glutamyl transpeptidase (gamma-GTP), an enzyme which converts LTC(4) to leukotriene D-4 (LTD(4)), and acts as an 'enzymatic barrier' to the vasoactive effects of LTC(4). Metabolism of LTD(4) in brain capillaries is, however, not known. In this study, rat renal dipeptidase (dehydropeptidase-I, microsomal dipeptidase; EC 3.4.13.11), which converts LTD(4) to leukotriene E(4) (LTE(4)) in kidney, was purified from rat kidney and the distribution of immunoreactive dipeptidase in multiple rat organs was determind. Immunocytochemicalmulti-organ analysis in the rat, which included brain, lung, heart, liver, spleen, small intestine, and testis, was performed. The antigen corresponding to renal dipeptidase was recognized in lung, liver, and testis. There was no antigen in the brain, heart, spleen, and small intestine. In order to confirm the absence of dipeptidase activity in brain capillaries, the metabolism of LTD, by isolated brain capillaries were examined by reversed phase high performance liquid chromatography. When LTD(4) was incubated with the isolated rat brain capillary, no measurable conversion of [H-3] LTD(4) to LTE(4) and leukotriene F-4 (LTF(4)) by brain capillaries was observed with 30 min of incubation. These findings suggest that although gamma-GTP acts as an enzymatic barrier and inactivates LTC(4), brain capillaries do not have metabolic activity against LTD(4).

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Documento generato il 30/11/20 alle ore 00:10:58