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Titolo:
ATRIAL-NATRIURETIC-PEPTIDE (ANP) AS A STIMULUS OF THE HUMAN ACROSOME REACTION AND A COMPONENT OF OVARIAN FOLLICULAR-FLUID - CORRELATION OF FOLLICULAR ANP CONTENT WITH IN-VITRO FERTILIZATION OUTCOME
Autore:
ANDERSON RA; FEATHERGILL KA; DRISDEL RC; RAWLINS RG; MACK SR; ZANEVELD LJD;
Indirizzi:
RUSH PRESBYTERIAN ST LUKES MED CTR,DEPT OBSTET & GYNECOL,1653 W COGRESS PKWY CHICAGO IL 60612 RUSH PRESBYTERIAN ST LUKES MED CTR,DEPT PHYSIOL CHICAGO IL 60612 RUSH PRESBYTERIAN ST LUKES MED CTR,DEPT BIOCHEM CHICAGO IL 60612 RUSH PRESBYTERIAN ST LUKES MED CTR,DEPT ANAT CHICAGO IL 60612
Titolo Testata:
Journal of andrology
fascicolo: 1, volume: 15, anno: 1994,
pagine: 61 - 70
SICI:
0196-3635(1994)15:1<61:A(AASO>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; HUMAN-SPERM CAPACITATION; GUANYLATE-CYCLASE; HUMAN-SPERMATOZOA; INVITRO CAPACITATION; CYCLIC-GMP; RECEPTOR; CELLS; PROGESTERONE; RAT;
Keywords:
NONCAPACITATED SPERMATOZOA; CAPACITATION; IN VITRO FERTILIZATION; GUANYLATE CYCLASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
R.A. Anderson et al., "ATRIAL-NATRIURETIC-PEPTIDE (ANP) AS A STIMULUS OF THE HUMAN ACROSOME REACTION AND A COMPONENT OF OVARIAN FOLLICULAR-FLUID - CORRELATION OF FOLLICULAR ANP CONTENT WITH IN-VITRO FERTILIZATION OUTCOME", Journal of andrology, 15(1), 1994, pp. 61-70

Abstract

Atrial natriuretic peptides (ANPs) from several species induced the human acrosome reaction. The maximal response was highest for human ANP(18.6% above unstimulated or baseline values) and decreased progressively for peptides derived from animals lower on the phylogenetic scale. ANP concentrations required for a half-maximal effect in noncapacitated spermatozoa ranged from 0.07 to 0.38 nM. ANP induced the acrosome reaction in capacitated spermatozoa, but the concentration required was higher than in noncapacitated cells. The response in noncapacitated spermatozoa was independent of added extracellular Ca2+ and was completely inhibited by 1 mu M LY83583 (inhibits particulate guanylate cyclase). However, 10 mu M N omega-nitro-L-arginine (inhibits soluble guanylate cyclase) had no effect. ANP (80 pM) and 3 mu M 1,2-dihexanoyl-sn-glycerol each induced a nearly half-maximal acrosome reaction. Added in combination, they produced no increased response, suggesting antagonism. Follicular fluid had variable levels of immunoreactive ANP. Average ANP content was nearly zero in samples that contained no oocyte at the time of aspiration but was higher (6.9 pM; 90% confidence limits =1.67-28.72 pM) in follicular fluid containing oocytes that did not fertilize in vitro. Highest concentrations of ANP were present in follicular fluid containing oocytes that fertilized in vitro (72.8 pM; 90% confidence limits = 38.1-139.1 pM). These data suggest that noncapacitated spermatozoa can acrosome react without added extracellular Ca2+ inresponse to an extracellular ligand. Also, human spermatozoa appear to contain receptors for ANP similar to those found in other cell types. The ANP content of follicular fluid might partly explain the abilityof follicular fluid to induce the acrosome reaction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 03:34:30