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Titolo:
IN-SITU ASSAY OF BASIC FIBROBLAST GROWTH-FACTOR IN CULTURED-CELLS WITH A SPECIFIC MONOCLONAL-ANTIBODY
Autore:
MINEMURA M; YOSHITAKE Y; MATSUZAKI K; WATANABE A; NISHIKAWA K;
Indirizzi:
KANAZAWA MED UNIV,DEPT BIOCHEM UCHINADA ISHIKAWA 92002 JAPAN KANAZAWA MED UNIV,DEPT BIOCHEM UCHINADA ISHIKAWA 92002 JAPAN W ALTON JONES CELL SCI CTR INC LAKE PLACID NY 12946 TOYAMA MED & PHARMACEUT UNIV,DEPT INTERNAL MED 3 TOYAMA 93001 JAPAN
Titolo Testata:
Cell structure and function
fascicolo: 5, volume: 18, anno: 1993,
pagine: 333 - 343
SICI:
0386-7196(1993)18:5<333:IAOBFG>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR PRODUCTION; CAPILLARY ENDOTHELIAL-CELLS; PROTEIN-FREE MEDIUM; EXTRACELLULAR-MATRIX; DNA-SYNTHESIS; NUCLEAR-LOCALIZATION; CARCINOMA-CELLS; BINDING; BRAIN; BFGF;
Keywords:
BASIC FIBROBLAST GROWTH FACTOR; CULTURED CELLS; MONOCLONAL ANTIBODY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
M. Minemura et al., "IN-SITU ASSAY OF BASIC FIBROBLAST GROWTH-FACTOR IN CULTURED-CELLS WITH A SPECIFIC MONOCLONAL-ANTIBODY", Cell structure and function, 18(5), 1993, pp. 333-343

Abstract

The localization of basic fibroblast growth factor (bFGF) in various cultured bFGF-producing cells, including bovine capillary endothelial (BCE) cells and human cholangiocellular carcinoma (HuCC-T1) cells, wasdetermined by measuring binding of I-125-labeled specific monoclonal antibody against bFGF. bFGF on the cell surface and within the cells was determined by counting the radioactivity of the labeled monoclonal antibody bound to the cells before and after increasing their permeability by treatment with alcohol or Triton X-100. The radioactivity bound to these cells decreased with increase in the concentration of unlabeled monoclonal antibody. Scatchard analyses of these data suggested the quantitative localization of bFGF and Kd values showing the specific binding. This method was designated as in situ assay with radio-labeled antibody (ISARA). bFGF detected on the cell surface and within thecells could be partially removed by treatment with heparin or heparinase and with heparin or DNase, respectively. Exogenous bFGF bound to cells not producing bFGF was also quantified by ISARA. Measurements of bFGF in extracts of cells producing bFGF suggested that 8.3-13.3% of the bFGF associated with the cells was detected by ISARA. This method should be useful for quantitative confirmation of immunohistochemical results on bFGF.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 16:15:04