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Titolo:
MODULATION OF LYMPHOHEMATOPOIESIS IN LONG-TERM CULTURES BY GAMMA-INTERFERON - DIRECT AND INDIRECT ACTION ON LYMPHOID AND STROMAL CELLS
Autore:
GIMBLE JM; MEDINA K; HUDSON J; ROBINSON M; KINCADE PW;
Indirizzi:
OKLAHOMA MED RES FDN,IMMUNOBIOL & CANC PROGRAM,825 NE 13TH ST OKLAHOMA CITY OK 73104
Titolo Testata:
Experimental hematology
fascicolo: 2, volume: 21, anno: 1993,
pagine: 224 - 230
SICI:
0301-472X(1993)21:2<224:MOLILC>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC GROWTH-FACTORS; COLONY-STIMULATING FACTOR; BONE-MARROW; RHEUMATOID-ARTHRITIS; IFN-GAMMA; PROGENITOR CELLS; APLASTIC-ANEMIA; GENE-EXPRESSION; MESSENGER-RNA; DOUBLE-BLIND;
Keywords:
IFN-GAMMA; LYMPHOHEMATOPOIESIS; STROMAL CELLS; LONG-TERM CULTURES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Gimble et al., "MODULATION OF LYMPHOHEMATOPOIESIS IN LONG-TERM CULTURES BY GAMMA-INTERFERON - DIRECT AND INDIRECT ACTION ON LYMPHOID AND STROMAL CELLS", Experimental hematology, 21(2), 1993, pp. 224-230

Abstract

Gamma interferon (IFN-gamma) is the product of multiple cell types within the bone marrow microenvironment and has been demonstrated to actas a potent inhibitor of myelopoiesis in vitro and in vivo. The action of this cytokine on lymphohemopoiesis has now been examined on both long-term bone marrow cultures and representative cloned cellular components of the bone marrow microenvironment. In myelopoietic (Dexter) cultures, the half maximal inhibitory concentration of IFN-gamma was between 1 and 10 U/mL. In comparable lymphopoietic (Whitlock/Witte) cultures, IFN-gamma inhibited the production of B-lineage lymphoid cells with a half maximal effective concentration of less than 1 U/mL. In a clonal assay for pre-B cells, IFN-gamma inhibited colony formation witha half maximal concentration of 1 to 5 U/mL. Not all B-lineage lymphoid cells displayed the same sensitivity, however. Growth of the IL-7-dependent B cell line (2E8) in methylcellulose assays was unaffected byIFN-gamma while the replication of other lymphoid lines was partiallyor completely inhibited. IFN-gamma induced the expression of cell surface proteins (MHC Class I and II) on both B-lineage cells and stromalcells. In cloned stromal cell lines, IFN-gamma increased the steady state mRNA levels for the cytokines interleukin-6 (IL-6) and JE, a member of the IL-8 family. These data indicate that IFN-gamma acts within the lymphohematopoietic microenvironment through both direct and indirect actions on the hemopoietic and stromal cell populations.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 11:30:54