Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
APPLICATION OF PCR TO DETECT NORWALK VIRUS IN FECAL SPECIMENS FROM OUTBREAKS OF GASTROENTERITIS
Autore:
MOE CL; GENTSCH TJ; ANDO T; GROHMANN G; MONROE SS; JIANG X; WANG J; ESTES MK; SETO Y; HUMPHREY C; STINE S; GLASS RI;
Indirizzi:
CTR DIS CONTROL & PREVENT,NATL CTR INFECT DIS,DIV VIRAL & RICKETTSIALDIS ATLANTA GA 30333 CTR DIS CONTROL & PREVENT,NATL CTR INFECT DIS,DIV VIRAL & RICKETTSIALDIS ATLANTA GA 30333 US FDA,OFF PLANT & DAIRY FOODS & BEVERAGES,DIV MICROANALYT EVALUAT WASHINGTON DC 20204 AUSTRALIAN WATER TECHNOL,WATER BOARD SYDNEY NSW AUSTRALIA BAYLOR COLL MED HOUSTON TX 77030 OSAKA INST PUBL HLTH & ENVIRONM SCI OSAKA OSAKA JAPAN
Titolo Testata:
Journal of clinical microbiology
fascicolo: 3, volume: 32, anno: 1994,
pagine: 642 - 648
SICI:
0095-1137(1994)32:3<642:AOPTDN>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNE ELECTRON-MICROSCOPY; POLYMERASE CHAIN-REACTION; REVERSE-TRANSCRIPTASE; AUSTRALIA; ANTIGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
C.L. Moe et al., "APPLICATION OF PCR TO DETECT NORWALK VIRUS IN FECAL SPECIMENS FROM OUTBREAKS OF GASTROENTERITIS", Journal of clinical microbiology, 32(3), 1994, pp. 642-648

Abstract

Norwalk virus (NV) and other small round-structured viruses (SRSVs) are frequent causes of gastroenteritis outbreaks. The recent cloning and sequencing of the NV genome has made it possible to detect NV and Norwalk-related viruses from fecal specimens by reverse transcription (RT)-PCR. We applied this technique to the examination of a total of 139fecal specimens from 19 outbreaks characterized by NV serology, including 56 samples from 7 NV outbreaks, 36 from 6 Norwalk-related virus outbreaks, and 47 from 6 outbreaks with SRSVs visualized by electron microscopy that were serologically unrelated to NV. Three primer pairs mere evaluated: two pairs in the polymerase region of NV and one pair near the 3' end of the genome. When one set of primers (primer pair 51-3) from the polymerase region was used, 40% of all samples were positive by RT-PCR and specimens from the NV outbreaks were more likely to be positive (64%) than those from outbreaks associated with Norwalk-related viruses (44%) or SRSVs (8%). To determine the relationship of theoutbreak strains to NV, we compared the sequences of a 145-base portion of the polymerase gene from 10 specimens obtained from five different outbreaks characterized as NV by serology. No two outbreak strains had the same sequence in this 145-base portion of the polymerase gene,and the identities of the nucleotide and amino acid sequences of these products compared crith the sequences of the corresponding region ofNV ranged from 62 to 79% and 69 to 90%, respectively. Because of sequence diversity in the polymerase region, the successful application ofRT-PCR to investigations of outbreaks of suspected NV-associated gastroenteritis will depend on the use of either multiple primer pairs or primers made against regions of the genome that are more conserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 10:13:18