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Titolo:
NEURONAL INTERFERON-GAMMA IMMUNOREACTIVE MOLECULE - BIOACTIVITIES ANDPURIFICATION
Autore:
OLSSON T; KELIC S; EDLUND C; BAKHIET M; HOJEBERG B; VANDERMEIDE PH; LJUNGDAHL A; KRISTENSSON K;
Indirizzi:
HUDDINGE UNIV HOSP,DEPT NEUROL S-14186 HUDDINGE SWEDEN KAROLINSKA INST,DEPT NEUROSCI,DIV NEURODEGENERAT STOCKHOLM SWEDEN TNO,INST APPL RADIOBIOL & IMMUNOL 2280 HV RIJSWIJK NETHERLANDS
Titolo Testata:
European Journal of Immunology
fascicolo: 2, volume: 24, anno: 1994,
pagine: 308 - 314
SICI:
0014-2980(1994)24:2<308:NIIM-B>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERIPHERAL NERVOUS-SYSTEM; RAT MOTOR NEURONS; CLASS-I ANTIGENS; MONOCLONAL-ANTIBODIES; TRYPANOSOMA-BRUCEI; INVIVO TREATMENT; MUSCLE CULTURES; FACIAL NUCLEUS; T-CELLS; EXPRESSION;
Keywords:
INTERFERON-GAMMA; MAJOR HISTOCOMPATIBILITY COMPLEX CLASS II; TRYPANOSOMA BRUCEI BRUCEI;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
T. Olsson et al., "NEURONAL INTERFERON-GAMMA IMMUNOREACTIVE MOLECULE - BIOACTIVITIES ANDPURIFICATION", European Journal of Immunology, 24(2), 1994, pp. 308-314

Abstract

An interferon (IFN)-gamma immunoreactive molecule, localized to smallneurons in peripheral sensory ganglia (N-IFN-gamma), has been detected with two mouse monoclonal antibodies (DB1 and DB16) directed againstdifferent epitopes of rat IFN-gamma. To define N-IFN-gamma with regard to its protein characteristics and bioactivities. DB1 and DB16 were used to purify N-IFN-gamma, from rat trigeminal ganglia in a two-step sequential antibody-affinity procedure. Sodium dodecylsulfate polyacrylamide gel electrophoresis (PAGE) and silver staining of purified N-IFN-gamma displayed three bands with an approximate molecular mass of 66, 62 and 54 kDa. The N-IFN-gamma, bioactivity was confined to the protein stained on gel when native material was run on PAGE, Biological effects of pure N-IFN-gamma were examined and compared with those of lymphocyte-derived recombinant IFN-gamma. N-IFN-gamma had antiviral effects in vitro and induced major histocompatibility complex class I and II antigens on macrophages and in cells in skeletal muscle cell cultures. N-IFN-gamma also stimulated myoblast proliferation and affected cholinergic receptor distribution on myotubes similar to recombinant IFN-gamma. Both molecules potently stimulated Trypanosoma brucei brucei growth. These data suggest that, although N-IFN-gamma, is a protein distinct from lymphocyte-derived IFN-gamma, the two molecules have enough structural similarities to allow for antibody recognition of at least two epitopes, and action on similar target structures on both parasiteand mammalian cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 15:12:04