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Titolo:
STABLE EXPRESSION OF BIOGENIC-AMINE TRANSPORTERS REVEALS DIFFERENCES IN INHIBITOR SENSITIVITY, KINETICS, AND ION DEPENDENCE
Autore:
GU H; WALL SC; RUDNICK G;
Indirizzi:
YALE UNIV,SCH MED,DEPT PHARMACOL NEW HAVEN CT 06510
Titolo Testata:
The Journal of biological chemistry
fascicolo: 10, volume: 269, anno: 1994,
pagine: 7124 - 7130
SICI:
0021-9258(1994)269:10<7124:SEOBTR>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLATELET SEROTONIN TRANSPORT; PLASMA-MEMBRANE VESICLES; DOPAMINE TRANSPORTER; NORADRENALINE TRANSPORT; CHLORIDE DEPENDENCE; ACTIVE-TRANSPORT; NEURONAL UPTAKE; RAT-BRAIN; GBR 12783; CLONING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
H. Gu et al., "STABLE EXPRESSION OF BIOGENIC-AMINE TRANSPORTERS REVEALS DIFFERENCES IN INHIBITOR SENSITIVITY, KINETICS, AND ION DEPENDENCE", The Journal of biological chemistry, 269(10), 1994, pp. 7124-7130

Abstract

We have constructed stable cell lines expressing transporters for dopamine (DA), norepinephrine (NE), and serotonin (5-HT) by transfection with cloned cDNAs. The parental LLC-PK1 cell does not express any of these neurotransmitter transporters. Therefore, monoamine transport activities in each of these cell lines are due to the transfected DNA only, allowing comparison in the same background. Drug inhibition profiles for each cell line are distinct and as expected for each transporter. LLC-NET and LLC-DAT cells transported both NE and DA and both cell types exhibited a lower K-M for DA transport than for NE transport. Analysis of V-max data for LLC-NET cells suggests that substrate is boundto the NE transporter during the rate-limiting step(s) in transport, The cocaine analog 2-beta carbomethoxy-3 beta-(4-[I-125]iodophenyl)tropane binds to each cell type, and is displaced by transport substrate in each case. Binding and transport measurements on parallel cell cultures allowed estimation of turnover numbers for norepinephrine, dopamine, and serotonin transporters. All three transporters require external Na+ and Cl-. The Na+ concentration dependence suggests that a singleNa+ ion is involved in transport catalyzed by norepinephrine and serotonin transporters while more than one Na+ ion participate in transport mediated by the dopamine transporter.

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Documento generato il 12/07/20 alle ore 01:44:43