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Titolo:
HUMAN-IMMUNODEFICIENCY-VIRUS ENV EXPRESSION BECOMES REV-INDEPENDENT IF THE ENV REGION IS NOT DEFINED AS AN INTRON
Autore:
HAMMARSKJOLD ML; LI HB; REKOSH D; PRASAD S;
Indirizzi:
UNIV VIRGINIA,MYLES H THALER CTR AIDS & HUMAN RETROVIRUS RES,HSC BOX 441,7-85 JORDAN HALL CHARLOTTESVILLE VA 22908 UNIV VIRGINIA,DEPT MICROBIOL CHARLOTTESVILLE VA 22908
Titolo Testata:
Journal of virology
fascicolo: 2, volume: 68, anno: 1994,
pagine: 951 - 958
SICI:
0022-538X(1994)68:2<951:HEEBRI>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
LATE REPLACEMENT VECTOR; STRUCTURAL GENE-EXPRESSION; TRANS-ACTIVATOR GENE; VIRAL MESSENGER-RNA; HIV-1 REV; HTLV-III; OVERLAP EXTENSION; PROTEIN; TYPE-1; SEQUENCES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
M.L. Hammarskjold et al., "HUMAN-IMMUNODEFICIENCY-VIRUS ENV EXPRESSION BECOMES REV-INDEPENDENT IF THE ENV REGION IS NOT DEFINED AS AN INTRON", Journal of virology, 68(2), 1994, pp. 951-958

Abstract

The human immunodeficiency virus (HIV) Rev protein functions to facilitate export of intron-containing HIV mRNA from the nucleus to the cytoplasm. We have previously shown that splice site recognition plays animportant role in Rev regulation of HIV env expression. Here we have further analyzed the effects of splice sites on HIV env expression andRev regulation, using a simian virus 40 late replacement vector system. env expression from the vector became completely Rev-independent when an excisable intron was positioned upstream of the env region, provided that env was not recognized as an intron. Complete Rev regulationwas restored either by the insertion of a 5' splice site between the intron and the env open reading frame or by deletion of the 3' splice site of the upstream intron. These results show that 5' splice sites can function as cis-acting represssor sequence (CRS) elements to retainRNA in the nucleus in the absence of Rev. They also indicate that Revregulation of HIV env expression is critically dependent on whether the env region is defined as an intron. This strengthens the hypothesisthat Rev interacts with components of the splicing machinery to release splicing factors and enable export of the mRNA before splicing occurs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 07:34:29