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Titolo:
INVOLVEMENT OF PROTEIN-KINASE-C DURING ACTIVATION OF THE MITOGEN-ACTIVATED PROTEIN-KINASE CASCADE BY LEUKEMIA INHIBITORY FACTOR - EVIDENCE FOR PARTICIPATION OF MULTIPLE SIGNALING PATHWAYS
Autore:
SCHIEMANN WP; NATHANSON NM;
Indirizzi:
UNIV WASHINGTON,DEPT PHARMACOL,SJ-30 SEATTLE WA 98195 UNIV WASHINGTON,DEPT PHARMACOL SEATTLE WA 98195
Titolo Testata:
The Journal of biological chemistry
fascicolo: 9, volume: 269, anno: 1994,
pagine: 6376 - 6382
SICI:
0021-9258(1994)269:9<6376:IOPDAO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR RECEPTOR; RIBOSOMAL PROTEIN-S6; MAP KINASE; GP130; TRANSDUCER; CELLS; PHOSPHORYLATION; EXPRESSION; LIF; RAS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
W.P. Schiemann e N.M. Nathanson, "INVOLVEMENT OF PROTEIN-KINASE-C DURING ACTIVATION OF THE MITOGEN-ACTIVATED PROTEIN-KINASE CASCADE BY LEUKEMIA INHIBITORY FACTOR - EVIDENCE FOR PARTICIPATION OF MULTIPLE SIGNALING PATHWAYS", The Journal of biological chemistry, 269(9), 1994, pp. 6376-6382

Abstract

We show here that treatment of 3T3-L1 cells with leukemia inhibitory factor (LIF) stimulates the activation of mitogen-activated protein kinase kinase (MAPKK), mitogen-activated protein kinase (MAPK), and S6 protein kinase (S6K) activities both in a time- and dose-dependent manner. A single peak of MAPKK activity, four peaks of activity against the S6 synthetic peptide, RRLSSLRA (S6 peptide), and three distinct peaks toward myelin basic protein (MBP) were observed after Mono Q chromatography of LIF-stimulated cell extracts. Two of the MBP kinase activities correlated with the stimulation of extracellular signal-regulated kinases 1 and 2. Interestingly, down-regulation of protein kinase C (PKC) by chronic treatment of 3T3-L1 cells with phorbol ester was found to attenuate, but not block, the LIF-mediated stimulation of MAPKK, MAPK, and S6K activities in 3T3-L1 cells. Treatment of 3T3-L1 cells withepidermal growth factor increased MAPKK, MAPK, and S6K activities to a similar extent as LIF, but this activation was not attenuated by down-regulation of PKC. Our results suggest that the full activation of the MAPK cascade by LIF may require inputs from multiple signaling pathways, one of which is dependent upon the presence of functional PKC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 19:28:01