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Titolo:
CHANGES IN THE GLYCOFORMS OF RAT ALPHA-1-ACID GLYCOPROTEIN DURING EXPERIMENTAL POLYARTHRITIS
Autore:
VENEMBRE PC; CONG HN; BIOU DR; DURAND GM;
Indirizzi:
UER SCI PHARMACEUT & BIOL,BIOCHIM GEN LAB,TOUR D4, 1ER ETAGE,5 RUE JBCLEMENT F-92296 CHATENAY MALABRY FRANCE UER SCI PHARMACEUT & BIOL,BIOCHIM GEN LAB F-92296 CHATENAY MALABRY FRANCE
Titolo Testata:
Clinica chimica acta
fascicolo: 1-2, volume: 221, anno: 1993,
pagine: 59 - 71
SICI:
0009-8981(1993)221:1-2<59:CITGOR>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
ASPARAGINE-LINKED OLIGOSACCHARIDES; GAS-LIQUID CHROMATOGRAPHY; PHASE PLASMA-PROTEINS; AFFINITY-CHROMATOGRAPHY; CONCANAVALIN-A; METHYL GLYCOSIDES; HEPATOMA-CELLS; INFLAMMATION; LIVER; INTERLEUKIN-1;
Keywords:
ALPHA-1-ACID GLYCOPROTEIN; INFLAMMATION; OLIGOSACCHARIDE; GLYCOFORM; RATS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
P.C. Venembre et al., "CHANGES IN THE GLYCOFORMS OF RAT ALPHA-1-ACID GLYCOPROTEIN DURING EXPERIMENTAL POLYARTHRITIS", Clinica chimica acta, 221(1-2), 1993, pp. 59-71

Abstract

We analyzed the carbohydrate moiety of purified alpha-1-acid glycoprotein (AGP) from Lewis adult male rats that were healthy (AGPh) or had experimental polyarthritis (AGPi). Sodium dodecyl sulfate polyacrylamide gel electrophoresis before and after N-glycanase treatment showed that AGPi had a slightly lower molecular mass (43 kDa vs. 45 kDa for AGPh) due to a lesser carbohydrate content. Carbohydrate analysis of purified AGP showed a slight decrease in the sialyl and galactosyl molar ratio in polyarthritis. However, the same difference in AGPh and AGPi (i.e. 0.6 residue) between the sialyl and galactosyl molar ratio indicated more than one sialyl residue per complex-type branch. Affinity for concanavalin A (ConA) of the whole glycoprotein and released oligosaccharides showed a progression during polyarthritis towards more reactive glycoforms or more ConA-bound oligosaccharides. Anion-exchange HPLC of the ConA-fractionated oligosaccharides corroborated the decreasedsialylation in polyarthritis. Taken together, these results suggest afall in branched and sialylated oligosaccharides during experimental polyarthritis. These structural changes might be related to an increase in Gal beta 1-4GlcNAc alpha 2-6 sialyltransferase activity describedelsewhere in inflammatory states.

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Documento generato il 13/07/20 alle ore 15:15:18