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Titolo:
INITIATION-FACTOR EIF-4E OF SACCHAROMYCES-CEREVISIAE - DISTRIBUTION WITHIN THE CELL, BINDING TO MESSENGER-RNA, AND CONSEQUENCES OF ITS OVERPRODUCTION
Autore:
LANG V; ZANCHIN NIT; LUNSDORF H; TUITE M; MCCARTHY JEG;
Indirizzi:
GESELL BIOTECHNOL FORSCH MBH,DEPT GENE EXPRESS,MASCHERODER WEG 1 D-38124 BRAUNSCHWEIG GERMANY GESELL BIOTECHNOL FORSCH MBH,DEPT GENE EXPRESS D-38124 BRAUNSCHWEIG GERMANY GESELL BIOTECHNOL FORSCH MBH,DEPT MICROBIOL D-38124 BRAUNSCHWEIG GERMANY UNIV KENT,BIOL LAB CANTERBURY KENT ENGLAND
Titolo Testata:
The Journal of biological chemistry
fascicolo: 8, volume: 269, anno: 1994,
pagine: 6117 - 6123
SICI:
0021-9258(1994)269:8<6117:IEOS-D>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; PROTEIN-SYNTHESIS; SECONDARY STRUCTURE; CAP RECOGNITION; DIRECTED MUTAGENESIS; ESCHERICHIA-COLI; CROSS-LINKING; YEAST; TRANSLATION; TRANSCRIPTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
V. Lang et al., "INITIATION-FACTOR EIF-4E OF SACCHAROMYCES-CEREVISIAE - DISTRIBUTION WITHIN THE CELL, BINDING TO MESSENGER-RNA, AND CONSEQUENCES OF ITS OVERPRODUCTION", The Journal of biological chemistry, 269(8), 1994, pp. 6117-6123

Abstract

The eukaryotic translational initiation factor 4E (eIF-4E) is an essential protein that binds the 5' cap structure with high specificity and affinity. Yeast eIF-4E is homologous to eIF-4E of higher eukaryotes,but interacts with a different set of cap-binding complex proteins. In the present study the distribution of yeast eIF-4E in Saccharomyces cerevisiae was found to be similar to that observed in higher cells, whereby the yeast factor was more concentrated in the nucleus than in the cytoplasm. Overexpression of yeast eIF-4E in S. cerevisiae exerted at most a minimal effect on growth in liquid minimal medium and was not found to influence the translation of reporter gene mRNAs bearing secondary structure in their leader regions. In a new method to study mRNA-protein interactions, biotinylated mRNAs were synthesized in vitro for use in studies of the binding of eIF-4E in yeast extracts. Streptavidin was used to adsorb the biotinylated mRNAs plus bound initiation factors. Stem-loop structures in the leader region did not influence the binding of eIF-4E or, in comparative experiments, of eIF-4A Thus yeast eIF-4E shows both similarities and differences with respect to thedistribution and function of its counterparts in higher eukaryotes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 20:01:14