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Titolo:
INHIBITION OF DESMIN EXPRESSION BLOCKS MYOBLAST FUSION AND INTERFERESWITH THE MYOGENIC REGULATORS MYOD AND MYOGENIN
Autore:
LI H; CHOUDHARY SK; MILNER DJ; MUNIR MI; KUISK IR; CAPETANAKI Y;
Indirizzi:
BAYLOR COLL MED,DEPT CELL BIOL HOUSTON TX 77030 BAYLOR COLL MED,DEPT CELL BIOL HOUSTON TX 77030
Titolo Testata:
The Journal of cell biology
fascicolo: 5, volume: 124, anno: 1994,
pagine: 827 - 841
SICI:
0021-9525(1994)124:5<827:IODEBM>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERMEDIATE FILAMENT PROTEINS; TISSUE-SPECIFIC EXPRESSION; TRANSFORMING GROWTH-FACTOR; MATRIX ATTACHMENT REGIONS; ANTISENSE RNA INHIBITION; DNA-BINDING PROTEIN; TRANSGENIC MICE; EPIDERMOLYTIC HYPERKERATOSIS; AVIAN ERYTHROCYTES; NUCLEAR-ENVELOPE;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
117
Recensione:
Indirizzi per estratti:
Citazione:
H. Li et al., "INHIBITION OF DESMIN EXPRESSION BLOCKS MYOBLAST FUSION AND INTERFERESWITH THE MYOGENIC REGULATORS MYOD AND MYOGENIN", The Journal of cell biology, 124(5), 1994, pp. 827-841

Abstract

The muscle-specific intermediate filament protein, desmin, is one of the earliest myogenic markers whose functional role during myogenic commitment and differentiation is unknown. Sequence comparison of the presently isolated and fully characterized mouse desmin cDNA clones revealed a single domain of polypeptide similarity between desmin and the basic and helix-loop-helix region of members of the myoD family myogenic regulators. This further substantiated the need to search for the function of desmin. Constructs designed to express anti-sense desmin RNA were used to obtain stably transfected (CC12)-C-2 myoblast cell lines. Several lines were obtained where expression of the anti-sense desmin RNA inhibited the expression of desmin RNA and protein down to basal levels. As a consequence, the differentiation of these myoblasts wasblocked; complete inhibition of myoblast fusion and myotube formationwas observed. Rescue of the normal phenotype was achieved either by spontaneous revertants, or by overexpression of the desmin sense RNA inthe defective cell lines. In several of the cell lines obtained, inhibition of desmin expression was followed by differential inhibition ofthe myogenic regulators myoD and/or myogenin, depending on the stage and extent of desmin inhibition in these cells. These data suggested that myogenesis is modulated by at least more than one pathway and desmin, which so far was believed to be merely an architectural protein, seems to play a key role in this process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 20:23:51