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Titolo:
MECHANISMS OF GASTRIC JUICE-INDUCED HYPERPERMEABILITY OF THE CULTUREDHUMAN TRACHEAL EPITHELIUM
Autore:
OHRUI T; YAMAYA M; SUZUKI T; SEKIZAWA K; FUNAYAMA T; SEKINE H; SASAKI H;
Indirizzi:
TOHOKU UNIV,SCH MED,DEPT GERIATR MED,AOBA KU,1-1 SEIRYO MACHI SENDAI MIYAGI 980 JAPAN TOHOKU UNIV,SCH MED,DEPT GERIATR MED,AOBA KU SENDAI MIYAGI 980 JAPAN TOHOKU UNIV,SCH MED,DEPT INTERNAL MED 3,AOBA KU SENDAI MIYAGI 980 JAPAN
Titolo Testata:
Chest
fascicolo: 2, volume: 111, anno: 1997,
pagine: 454 - 459
SICI:
0012-3692(1997)111:2<454:MOGJHO>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
AIRWAY ION-TRANSPORT; ASPIRATION; PERMEABILITY; PH;
Keywords:
ASPIRATION PNEUMONIA; GASTRIC JUICE; OSMOLARITY; PEPSIN; PH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
T. Ohrui et al., "MECHANISMS OF GASTRIC JUICE-INDUCED HYPERPERMEABILITY OF THE CULTUREDHUMAN TRACHEAL EPITHELIUM", Chest, 111(2), 1997, pp. 454-459

Abstract

Purpose: The respiratory aspiration of the stomach contents causes severe lung damage called aspiration pneumonia. The present study was undertaken to elucidate whether mucosal exposure of gastric juice causeshyperpermeability of the human airway epithelium and to determine themechanisms responsible for gastric juice-induced airway epithelial damage. Materials and methods: Gastric juice was collected from 46 normal adults via gastroscope and samples were analyzed for pH, osmolarity,and concentration of pepsin and trypsin. Tracheal surface epithelial cells were obtained from 16 autopsies, cultured onto porous filters, and mounted in the Ussing chamber, Electrical conductance (G) was measured before and after exposure of cells to gastric juice or Krebs-Henseleit solution with pH at 1.8, 2.8, 4.0, or 7.4 in the presence or absence of pepsin. D-[H-3] mannitol flux study across the epithelial layerand histologic observations using an inverted microscope were also performed after exposure of cells to gastric juice. Results: Exposure ofcultured human tracheal epithelium to gastric juice caused increases in G in a time- and pH-dependent fashion, A pepsin inhibitor (pepstatin A) inhibited gastric juice-induced increases in G at a pH of 2.8, and the addition of pepsin augmented increases in G induced by the Krebs-Henseleit solution at a pH of 1.8 and 2.8. Lowering the osmolarity ofthe solution to levels similar to gastric juice also potentiated increases in G induced by acid and pepsin. Gastric juice caused increases in D-[H-3] mannitol flux across the epithelial layer bidirectionally, and microscopic observation revealed separation of the intercellular space and cell detachment from culture vessels after exposure of cells to gastric juice. Conclusion: Gastric juice causes hyperpemeability across human airway epithelium probably through the additive effects of gastric acid, pepsin activity, and lower osmolarity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 04:20:33