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Titolo:
DETECTION OF OVARIAN-CANCER BY AU-198-LABELED HUMAN MONOCLONAL-ANTIBODY
Autore:
CHAUDHURI TR; ZINN KR; MORRIS JS; MCDONALD GA; LLORENS AS; CHAUDHURI TK;
Indirizzi:
UNIV MISSOURI,RES REACTOR COLUMBIA MO 65211 UNIV MISSOURI,DEPT MOLEC MICROBIOL & IMMUNOL COLUMBIA MO 00000 UNIV MISSOURI,DEPT GYNECOL ONCOL COLUMBIA MO 00000 UNIV TEXAS,HLTH SCI CTR SAN ANTONIO TX 00000
Titolo Testata:
Cancer
fascicolo: 3, volume: 73, anno: 1994, supplemento:, S
pagine: 878 - 883
SICI:
0008-543X(1994)73:3<878:DOOBAH>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTRON-MICROSCOPY; COLLOIDAL GOLD; PROTEIN-A; ANTIGEN; CARCINOMA; REAGENT; LABEL;
Keywords:
HUMAN MONOCLONAL ANTIBODY; OVARIAN CANCER; AU-198; AU-198; RADIOIMMUNODIAGNOSIS; RADIOLABELING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
T.R. Chaudhuri et al., "DETECTION OF OVARIAN-CANCER BY AU-198-LABELED HUMAN MONOCLONAL-ANTIBODY", Cancer, 73(3), 1994, pp. 878-883

Abstract

Background. There is no reliable method for the early diagnosis of ovarian cancer. Radiolabeled monoclonal antibodies have potential to assist in early diagnosis, but they are limited by problems that include antibody specificity, stability, and immunoreactivity, as well as patient reactions to the antibodies used. Methods. Methods were developed to Au-198-label a human monoclonal antibody (TC5 antibody), developed against an ovarian cancer cell surface antigen. Antigen binding sites on the TC5 antibody were protected with sepharose 4B affinity chromatography before Au-198-labeling. The Au-198-labeled TC5 antibody was evaluated with biopsy specimens in a blind study. The immunoreactivity ofradiolabeled TC5 antibody also was evaluated in slot-blot experimentswith extracts of the biopsy specimens. Results. The Au-198-labeled TC5 antibody had high binding reaction to all biopsy specimens (six of six) pathologically diagnosed as ovarian cancer (serous and endometrioid adenocarcinoma). The radiolabeled TC5 antibody did not bind to any normal (non-neoplastic) specimens (zero in ten), with one exception. One ''normal'' ovary specimen had high binding of radiolabeled TC5 antibody, and metastatic ovarian cancer was diagnosed 4 months later. The TC5 antibody labeled with Au-198, without protecting antigen-binding sites, did not bind to any biopsy specimens. Conclusions. The affinity-labeling method was necessary to protect antigen-binding sites and preserve the immunoreactivity of the TC5 antibody. The Au-198-labeling method may be an ideal technique to evaluate monoclonal antibodies in vitro. The TC5 antibody had high sensitivity and specificity for detecting ovarian cancer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/08/20 alle ore 08:35:05