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Titolo:
HYPOTENSION IN TRANSGENIC MICE OVEREXPRESSING HUMAN BRADYKININ B-2 RECEPTOR
Autore:
WANG DZ; CHAO L; CHAO J;
Indirizzi:
MED UNIV S CAROLINA,DEPT BIOCHEM & MOL BIOL,171 ASHLEY AVE CHARLESTONSC 29425 MED UNIV S CAROLINA,DEPT BIOCHEM & MOL BIOL CHARLESTON SC 29425
Titolo Testata:
Hypertension
fascicolo: 1, volume: 29, anno: 1997,
parte:, 2
pagine: 488 - 493
SICI:
0194-911X(1997)29:1<488:HITMOH>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPONTANEOUSLY HYPERTENSIVE RATS; SEQUENCE-ANALYSIS; BLOOD-PRESSURE; GENE; CLONING; EXPRESSION; KININS; CELLS; SITES;
Keywords:
HUMAN BRADYKININ B-2 RECEPTOR; TRANSGENIC MICE; HYPOTENSION; HOE 140; UTERINE CONTRACTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
D.Z. Wang et al., "HYPOTENSION IN TRANSGENIC MICE OVEREXPRESSING HUMAN BRADYKININ B-2 RECEPTOR", Hypertension, 29(1), 1997, pp. 488-493

Abstract

Bradykinin binds to its receptor at target organs and exerts a wide spectrum of biological activities including vasodilation, smooth musclecontraction and relaxation, pain, and inflammation. To gain a better insight into the physiological function of this potent vasoactive peptide, we created transgenic mice that harbor the human bradykinin B-2 receptor transgene under the control of the Rous sarcoma virus 3'-LTR promoter (RSV-cHBKR). Expression of HBKR in these transgenic mice was identified in the aorta, brain, heart, lung, liver, kidney, uterus, andprostate gland by reverse transcription-polymerase chain reaction Southern blot analysis. Two transgenic mouse lines expressing the human B-2 receptor resulted in a significant reduction of blood pressure (84.2 +/- 0.6 mm Hg, n = 28, 76.9 +/- 0.8 mm Hg, n = 24, P < .001) compared with the control littermates (96.9 +/- 0.4 mm Hg, n = 52 ). Administration of Hoe 140, a bradykinin B-2 receptor antagonist, restored the blood pressure of the transgenic mice to normal levels within 1 hour, and the effect diminished within 4 hours. The transgenic mice displayed enhanced blood pressure-lowering effect induced by a bolus intra-aortic injection of kinin and showed increased response in kinin-induced uterine smooth muscle contractility compared with control littermates. These studies show that overexpression of human bradykinin B-2 receptor causes a sustained reduction of blood pressure in transgenic mice. They also suggest that the B-2 receptor-mediated signal transduction pathway plays a role in blood pressure regulation.

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Documento generato il 23/11/20 alle ore 20:03:38