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Titolo:
CHARACTERIZATION OF PREVOTELLA-INTERMEDIA AND PREVOTELLA-NIGRESCENS ISOLATES FROM PERIODONTIC AND ENDODONTIC INFECTIONS
Autore:
GHARBIA SE; HAAPASALO M; SHAH HN; KOTIRANTA A; LOUNATMAA K; PEARCE MA; DEVINE DA;
Indirizzi:
UNIV LONDON,EASTMAN DENT HOSP,DEPT MICROBIOL,256 GRAYS INN RD LONDON WC1X 8LD ENGLAND DALHOUSIE UNIV,FAC DENT HALIFAX NS CANADA UNIV HELSINKI,DEPT CARIOL HELSINKI FINLAND UNIV BRADFORD,DEPT BIOMED SCI BRADFORD BD7 1DP W YORKSHIRE ENGLAND
Titolo Testata:
Journal of periodontology
fascicolo: 1, volume: 65, anno: 1994,
pagine: 56 - 61
SICI:
0022-3492(1994)65:1<56:COPAPI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLACK-PIGMENTED BACTEROIDES; SP-NOV; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; EPITHELIAL-CELLS; GINGIVALIS; ASACCHAROLYTICUS; ADHERENCE; PROPOSAL; BACTERIA; DISEASE;
Keywords:
PERIODONTAL DISEASES PATHOGENESIS; PERIODONTAL DISEASES MICROBIOLOGY; DENTAL PULP DISEASES MICROBIOLOGY; PREVOTELLA INTERMEDIA; PREVOTELLA NIGRESCENS; DENTAL PULP DISEASES PATHOGENESIS; SURFACE PROPERTIES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
S.E. Gharbia et al., "CHARACTERIZATION OF PREVOTELLA-INTERMEDIA AND PREVOTELLA-NIGRESCENS ISOLATES FROM PERIODONTIC AND ENDODONTIC INFECTIONS", Journal of periodontology, 65(1), 1994, pp. 56-61

Abstract

THE OCCURRENCE AND SURFACE PROPERTIES Of Prevotella intermedia and P.nigrescens in healthy sites and in periodontic and endodontic infections were studied among 73 strains, tentatively identified as P. intermedia. Fifteen strains were from necrotic root canal infections, 41 were from periodontal samples, and 17 isolates were obtained from healthygingival sites. Identification of isolates as either P. intermedia orP. nigrescens was based on differences in malate and glutamate dehydrogenase electrophoretic mobilities which allowed unambiguous separation of P. intermedia and P. nigrescens. The majority of strains from periodontal samples were P. intermedia (29 of 41 strains). In endodontic samples only 4 out of 15 isolates were P. intermedia, while all except1 of 17 strains from healthy gingival sites were identified as P. nigrescens. SDS-PAGE of whole cell proteins revealed 31 and 38 kDa proteins in P. nigrescens which were not detected in P. intermedia. Surface biotinylation of cells, followed by Western blotting and detection by alkaline phosphatase conjugated extravidin, showed strong staining of the 31 kDa protein in P. nigrescens indicating that this protein is located on the surface of the cell. Corresponding staining was not seen in P. intermedia. Fimbria-like projections were observed using electron microscopy of negatively-stained cells of P. nigrescens. The resultsshow that P. intermedia and P. nigrescens may have different site specificities and surface properties and thus emphasize the need for accurate identification of these two species for the evaluation of their role in the pathogenesis of oral infections.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 06:50:00