Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ACETALDEHYDE ACTIVATES THE PROMOTER OF THE MOUSE ALPHA(2)(I) COLLAGENGENE
Autore:
PARES A; POTTER JJ; RENNIE L; MEZEY E;
Indirizzi:
JOHNS HOPKINS UNIV,SCH MED,DEPT MED,921 ROSS RES BLDG,720 RUTLAND AVEBALTIMORE MD 21205
Titolo Testata:
Hepatology
fascicolo: 2, volume: 19, anno: 1994,
pagine: 498 - 503
SICI:
0270-9139(1994)19:2<498:AATPOT>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH FACTOR-BETA; FAT-STORING CELLS; ISOLATED RAT HEPATOCYTES; NUCLEAR FACTOR-I; STIMULATES COLLAGEN; ETHANOL-METABOLISM; HUMAN-FIBROBLASTS; BINDING-FACTOR; DNA-BINDING; TRANSCRIPTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
A. Pares et al., "ACETALDEHYDE ACTIVATES THE PROMOTER OF THE MOUSE ALPHA(2)(I) COLLAGENGENE", Hepatology, 19(2), 1994, pp. 498-503

Abstract

The mechanism whereby ethanol ingestion results in hepatic fibrosis remains unknown. Acetaldehyde has been shown to increase alpha(1)(I) collagen gene transcription in human fibroblasts and in rat myofibroblastlike cells (Ito cells) in culture. In this study, the effect of acetaldehyde was determined on the activation of the alpha(2)(I) collagen promoter. A plasmid containing the mouse alpha(2)(I) collagen promoter region (-2000 to 54), fused to the coding sequence of the reporter gene chloramphenicol acetyl transferase and similar plasmid constructs containing deletions in the collagen promoter, were transfected into NIH3T3 fibroblasts in culture. Acetaldehyde (200 mu mol/L) and transforming growth factor-beta 1 (5 ng/ml) activated the wild type promoter. The combination of acetaldehyde and transforming growth factor-beta 1 did not result in a greater effect than either alone. Acetaldehyde inhibited, whereas transforming growth factor-beta 1 did not activate, thepromoter, with a -352 to -104 deletion. By contrast, acetaldehyde hadno effect, whereas transforming growth factor-beta 1 resulted in a small decrease in the activity of the promoter, with a -501 to -352 deletion. This study shows that acetaldehyde and transforming growth factor-beta 1 independently activate the mouse alpha(2)(I) collagen promoter and that this activation is mediated by the same proximal region of the promoter.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 22:18:54