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Titolo:
GENOTOXICITY OF N-NITROSO-N-METHYLUREA AND ACETONE OXIME IN THE TRANSGENIC DROSOPHILA CARRYING THE HUMAN GENE ENCODING A SUBUNIT OF GLUTATHIONE-S-TRANSFERASE
Autore:
RYSKOVA M; CHROUST K; TRBUSEK M; BENEDIK J; JOWETT T;
Indirizzi:
MASARYK UNIV,FAC SCI,DEPT MOL BIOL & GENET,KOTLARSKA 2 CZ-61137 BRNO CZECH REPUBLIC MASARYK UNIV,FAC SCI,DEPT MOL BIOL & GENET CZ-61137 BRNO CZECH REPUBLIC UNIV NEWCASTLE,SCH MED,DEPT BIOCHEM & GENET NEWCASTLE TYNE TYNE & WEAR ENGLAND
Titolo Testata:
Folia biologica
fascicolo: 1, volume: 43, anno: 1997,
pagine: 19 - 24
SICI:
0015-5500(1997)43:1<19:GONAAO>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
MELANOGASTER;
Keywords:
TRANSGENIC DROSOPHILA MELANOGASTER; WING SPOT TEST; SMART; N-NITROSO-N-METHYLUREA; ACETONE OXIME; GLUTATHIONE S-TRANSFERASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
11
Recensione:
Indirizzi per estratti:
Citazione:
M. Ryskova et al., "GENOTOXICITY OF N-NITROSO-N-METHYLUREA AND ACETONE OXIME IN THE TRANSGENIC DROSOPHILA CARRYING THE HUMAN GENE ENCODING A SUBUNIT OF GLUTATHIONE-S-TRANSFERASE", Folia biologica, 43(1), 1997, pp. 19-24

Abstract

The genotoxic effects of N-nitroso-N-methylurea (MNU) and acetone oxime (ACOX) were tested in the Somatic Mutation and Recombination Test (SMART) in Drosophila melanogaster. We have performed the same assay ontransgenic flies expressing the human gene encoding a glutathione S-transferase alpha subunit (HGST), The SMART assay is used here to demonstrate genotoxicity and to determine the effect of human glutathione S-transferase on the genotoxic response, Three types of Drosophila strains were used: non-transgenic strains first described by Szabad (1986), transgenic strains derived from the Szabad strains but expressing the bacterial lacZ gene, and similarly derived transgenic strains expressing the HGST gene, MNU was highly genotoxic in both transgenic and non-transgenic flies. The non-transgenic flies were significantly more sensitive to the genotoxic effects of MNU compared to both types of transgenic flies, There were statistically significant differences between the transgenic HGST crosses and transgenic lacZ and non-transgenic control crosses but there was no significant difference between the genotoxic response to MNU in flies from the transgenic cross with lacZ and from the cross carrying three copies of HGST. ACOX also proved to begenotoxic to both non-transgenic and transgenic flies. However, fliescarrying three copies of the gene were significantly more resistant to the genotoxic effect of ACOX than those transgenic flies with two orno copies of the human gene.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 07:13:44