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Titolo:
REGULATION ANALYSIS OF ENERGY-METABOLISM
Autore:
BRAND MD;
Indirizzi:
UNIV CAMBRIDGE,DEPT BIOCHEM,TENNIS COURT RD CAMBRIDGE CB2 1QW ENGLAND
Titolo Testata:
Journal of Experimental Biology
fascicolo: 2, volume: 200, anno: 1997,
pagine: 193 - 202
SICI:
0022-0949(1997)200:2<193:RAOE>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
POTATO-TUBER MITOCHONDRIA; DOWN ELASTICITY ANALYSIS; RAT-LIVER MITOCHONDRIA; OXIDATIVE-PHOSPHORYLATION; STEADY-STATE; PROTON LEAK; CONTROL COEFFICIENTS; ATP TURNOVER; RESPIRATION; SYSTEMS;
Keywords:
STEADY STATE; REGULATION; HOMEOSTASIS; REGULATION ANALYSIS; METABOLIC CONTROL ANALYSIS; RESPONSE COEFFICIENT; OXIDATIVE PHOSPHORYLATION; ENERGY METABOLISM; MITOCHONDRIA; CADMIUM; HEPATOCYTES; MUSCLE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
M.D. Brand, "REGULATION ANALYSIS OF ENERGY-METABOLISM", Journal of Experimental Biology, 200(2), 1997, pp. 193-202

Abstract

This paper reviews top-down regulation analysis, a part of metabolic control analysis, and shows how it can be used to analyse steady states, regulation and homeostasis in complex systems such as energy metabolism in mitochondria, cells and tissues. A steady state is maintained by the variables in a system; regulation is the way the steady state is changed by external effecters. We can exploit the properties of the steady state to measure the kinetic responses (elasticities) of reactions to the concentrations of intermediates and effecters, We can reduce the complexity of the system under investigation by grouping reactions into large blocks connected by a small number of explicit intermediates - this is the top-down approach to control analysis, Simple titrations then yield all the values of elasticities and control coefficients within the system, We can use these values to quantify the relativestrengths of different internal pathways that act to keep an intermediate or a rate constant in the steady state, We can also use them to quantify the relative strengths of different primary actions of an external effector and the different internal pathways that transmit its effects through the system, to describe regulation and homeostasis. Thistop-down regulation analysis has been used to analyse steady states of energy metabolism in mitochondria, cells and tissues, and to analyseregulation of energy metabolism by cadmium, an external effector, in mitochondria. The combination of relatively simple experiments and newtheoretical structures for presenting and interpreting the results means that top-down regulation analysis provides a novel and effective way to analyse steady states, regulation and homeostasis in intricate metabolic systems.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 05:25:57