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Titolo:
GABA TRANSPORT AND SUBCELLULAR-DISTRIBUTION IN THE RAT ANTERIOR-PITUITARY GLAND
Autore:
DUVILANSKI B; SEILICOVICH A; DEBELJUK L; LASAGA M; DIAZ MD; PISERA D;
Indirizzi:
UNIV BUENOS AIRES,FAC MED,CTR INVEST REPROD,PISO 10 RA-1121 BUENOS AIRES ARGENTINA
Titolo Testata:
Neuroendocrinology
fascicolo: 2, volume: 59, anno: 1994,
pagine: 183 - 188
SICI:
0028-3835(1994)59:2<183:GTASIT>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-AMINOBUTYRIC ACID; PROLACTIN SECRETION; GABAERGIC SYSTEM; MEDIAN-EMINENCE; RELEASE; DECARBOXYLASE; HYPOTHALAMUS; INHIBITION; RECEPTORS; INVITRO;
Keywords:
GABA UPTAKE; ANTERIOR PITUITARY; SUBCELLULAR DISTRIBUTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
B. Duvilanski et al., "GABA TRANSPORT AND SUBCELLULAR-DISTRIBUTION IN THE RAT ANTERIOR-PITUITARY GLAND", Neuroendocrinology, 59(2), 1994, pp. 183-188

Abstract

In this investigation we have studied the uptake of gamma aminobutyric acid (GABA) into anterior pituitary slices. Tissue:medium ratios of about 45:1 were obtained after a 30-min incubation. The process responsible for H-3-GABA uptake was temperature-sensitive and sodium-dependent. The kinetic constants of saturable GABA transport were: K-m 4.141 mu M and V-max 0.973 pmol/min/mg protein, at 25 degrees C. The incorporation of GABA into anterior pituitary was inhibited by specific inhibitors of neuronal and/or glial uptake. The subcellular distribution ofGABA was investigated by continuous sucrose density gradients and differential centrifugation. Most of the endogenous and labelled GABA waspresent in the soluble fraction. However, a small part of GABA was found in the particulate fraction. These observations indicate that the anterior pituitary gland is able to concentrate GABA which interacts with intracellular particles.

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Documento generato il 02/04/20 alle ore 08:58:17