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Titolo:
SEQUENCES OF WILD PUUMALA VIRUS GENES SHOW A CORRELATION OF GENETIC-VARIATION WITH GEOGRAPHIC ORIGIN OF THE STRAINS
Autore:
PLYUSNIN A; VAPALAHTI O; ULFVES K; LEHVASLAIHO H; APEKINA N; GAVRILOVSKAYA I; BLINOV V; VAHERI A;
Indirizzi:
UNIV HELSINKI,DEPT VIROL,POB 21,HAARTMANINKATU 3 SF-00014 HELSINKI FINLAND MOSCOW POLIOMYELITIS & VIRAL ENCEPHALITIS RES INS MOSCOW RUSSIA KOLTSOVO MOLEC BIOL RES INST KOLTSOV RUSSIA
Titolo Testata:
Journal of General Virology
, volume: 75, anno: 1994,
parte:, 2
pagine: 405 - 409
SICI:
0022-1317(1994)75:<405:SOWPVG>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEPHROPATHIA EPIDEMICA; BANK VOLES; M-GENOME; HANTAVIRUSES; EXPRESSION; DIAGNOSIS; INFECTION; SEGMENTS; CLONING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
A. Plyusnin et al., "SEQUENCES OF WILD PUUMALA VIRUS GENES SHOW A CORRELATION OF GENETIC-VARIATION WITH GEOGRAPHIC ORIGIN OF THE STRAINS", Journal of General Virology, 75, 1994, pp. 405-409

Abstract

An experimental scheme was developed for direct sequence analysis of Puumala virus-containing specimens from wild rodents (Clethrionomys glareolus). Total RNA isolated from rodent lung tissues was reverse-transcribed in the presence of a universal 11 nucleotide primer complementary to all three viral RNA segments followed by amplification in a PCRwith gene-specific primers. A full-length PCR product of approximately 1800 bp from the S segment encoding the viral nucleoprotein and a product of approximately 900 bp from the M segment (encoding the C-terminal two-thirds of the G2 protein and including the 3' non-coding region) of Puumala virus (from C. glareolus trapped in Udmurtia) were prepared and sequenced. No pronounced differences to Vero cell-grown viruses were seen. The Udmurtia/894Cg/91 strain was more closely related to the Bashkiria/CG18-20/84 strain than to the Finnish prototype strain of Puumala virus, Sotkamo/V-2969/81. Thus there is a correlation with the geographic origin of the three strains. The results indicate the occurrence of genetic drift and different selection pressures leading to(i) clustering of mutations, (ii) a lower frequency of nucleotide substitutions in the coding than in the 3' noncoding regions and (iii) a higher frequency of amino acid substitutions in G2 than in the N protein.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/10/20 alle ore 22:00:08