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Titolo:
IN-VITRO EFFECTS OF GLUCOSE POLYMER-CONTAINING PERITONEAL-DIALYSIS FLUIDS ON PHAGOCYTIC-ACTIVITY
Autore:
THOMAS S; SCHENK U; FISCHER FP; METTANG T; PASSLICKDEETJEN J; KUHLMANN U;
Indirizzi:
ROBERT BOSCH KRANKENHAUS,DEPT NEPHROL,AUERBACHSTR 110 D-70376 STUTTGART GERMANY ROBERT BOSCH KRANKENHAUS,DEPT NEPHROL D-70376 STUTTGART GERMANY FRESENIUS AG OBERURSEL GERMANY
Titolo Testata:
American journal of kidney diseases
fascicolo: 2, volume: 29, anno: 1997,
pagine: 246 - 253
SICI:
0272-6386(1997)29:2<246:IEOGPP>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAPD; BIOCOMPATIBILITY; MULTICENTER; ICODEXTRIN; LEUKOCYTES; INVITRO;
Keywords:
PERITONEAL DIALYSIS; CONTINUOUS AMBULATORY PERITONEAL DIALYSIS; GLUCOSE POLYMER; OSMOLARITY; BIOCOMPATIBILITY; PHAGOCYTIC ACTIVITY; HOST DEFENSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
S. Thomas et al., "IN-VITRO EFFECTS OF GLUCOSE POLYMER-CONTAINING PERITONEAL-DIALYSIS FLUIDS ON PHAGOCYTIC-ACTIVITY", American journal of kidney diseases, 29(2), 1997, pp. 246-253

Abstract

Commercially available peritoneal dialysis fluids (PDFs) are known toimpair peritoneal cellular defense mechanisms. We have investigated the influence of glucose polymer-containing PDFs on phagocytic functionin vitro. Polymorphonuclear neutrophils (PMNLs) and monocytes (MNs) from 10 continuous ambulatory peritoneal dialysis patients and 10 healthy donors were incubated in PDFs containing either 7.5% icodextrin (glucose polymer) or 1.5% glucose at original pH and pH 7.4. Chemiluminescence response and H2O2 production were measured following stimulationwith preopsonized Staphylococcus epidermidis or phorbol myristate acetate. Phagocytosis of radiolabeled bacteria and killing capacity of the cells were determined. A comparison of the impact of glucose polymerversus glucose-containing solutions at their original pH on the oxidative metabolism of the cells showed a highly significant difference (P< 0.0001) in favor of glucose polymers for H2O2 production of PMNLs (7.78 +/- 4.5 nmol cytochrome C reduction/10(6) cells/min v 1.11 +/- 0.67 nmol cytochrome C reduction/10(6) cells/min) and MNs (7.66 +/- 3.6 nmol cytochrome C reduction/10(6) cells/min v 1.29 +/- 0.86 nmol cytochrome C reduction/10(6) cells/min). Correspondingly, PMNLs and MNs incubated in glucose polymers showed a significantly higher chemiluminescence response irrespective of the stimulant used (P < 0.0001). Applying the killing assay on PMNLs also revealed a significantly higher percentage of inactivated bacteria (45.5% +/- 11.0% v 29.2% +/- 15.5%; P <0.05), After adjustment of pH to 7.4, a significant difference could only be found for H2O2 production of PMNLs in favor of glucose polymers (16.73 +/- 6.98 nmol cytochrome C reduction/10(6) cells/min v 11.65 /- 5.37 nmol cytochrome C reduction/10(6) cells/min; P < 0.05). In addition, we compared the glucose-polymer solution to an otherwise equally composed equiosmolar solution that contained 0.274% glucose insteadof glucose polymers. No significant differences were detected with any of the tests applied. Our data suggest that glucose polymer solutions are comparatively less suppressive to phagocytic function than currently used glucose-containing PDFs. This effect may be attributed to the low osmolarity of these solutions. (C) 1997 by the National Kidney Foundation, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 10:17:22