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Titolo:
CHARACTERIZATION AND AMINO-TERMINAL SEQUENCE OF PHOSPHOLIPASE A(2)-IIFROM THE VENOM OF AGKISTRODON BILINEATUS (COMMON CANTIL)
Autore:
NIKAI T; KOMORI Y; OHARA A; YAGIHASHI S; OHIZUMI Y; SUGIHARA H;
Indirizzi:
MEIJO UNIV,FAC PHARM,DEPT MICROBIOL,TENPAKU KU,YAGOTOYAMA 150 NAGOYA AICHI 468 JAPAN MEIJO UNIV,FAC PHARM,DEPT MICROBIOL,TENPAKU KU NAGOYA AICHI 468 JAPAN NISSUI PHARMACEUT CO LTD,INST DIAGNOST REAGENTS YUKI IBARAKI 307 JAPAN TOHOKU UNIV,INST PHARMACEUT SENDAI MIYAGI 980 JAPAN
Titolo Testata:
International Journal of Biochemistry
fascicolo: 1, volume: 26, anno: 1994,
pagine: 43 - 48
SICI:
0020-711X(1994)26:1<43:CAASOP>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
PURIFICATION; SNAKE; A2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
16
Recensione:
Indirizzi per estratti:
Citazione:
T. Nikai et al., "CHARACTERIZATION AND AMINO-TERMINAL SEQUENCE OF PHOSPHOLIPASE A(2)-IIFROM THE VENOM OF AGKISTRODON BILINEATUS (COMMON CANTIL)", International Journal of Biochemistry, 26(1), 1994, pp. 43-48

Abstract

1. Phospholipase A(2) was isolated from the venom of Agkistrodon bilineatus by Sephadex G-75 and CM-Cellulose column chromatographies. 2. The purified phospholipase A(2) gave a single band on disc polyacrylamide gel electrophoresis, sodium dodecyl sulfate polyacrylamide gel electrophoresis and ODS-HPLC. 3. The enzyme preparation had a mel. wt of 14,000, isoelectric point of pH 10.12 and possessed 121 amino acid residues. 4. The enzyme hydrolyzed the phospholipids phosphatidyl choline,phosphatidyl ethanolamine, phosphatidyl inositol and phosphatidyl serine. 5. The contraction of mouse diaphragm was inhibited by phospholipase A(2)-II. 6. Phospholipase A(2) activity of this preparation was inhibited by ethylenediamine tetraacetic acid, ethlyleneglycol (beta-aminoethyl) N,N,N',N'-tetraacetic acid, p-bromophenacyl bromide or N-bromosuccinimide, but not by iodoacetic acid or diisopropyl fluorophosphate. 7. The amino-terminal sequence of the PLA(2)-II was determined.

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Documento generato il 30/03/20 alle ore 09:24:44