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Titolo:
OSTEOCLAST DEVELOPMENT IN THE COCULTURE SYSTEM OF PERIOSTLESS METATARSAL BONES AND HEMATOPOIETIC-CELLS STUDIED BY IN-SITU HYBRIDIZATION WITH A PROBE FOR Y-CHROMOSOMES
Autore:
HAGENAARS CE; KAWILARANGDEHAAS EWM; HAZEKAMP J; WIEGANT J; NIJWEIDE PJ;
Indirizzi:
LEIDEN UNIV,CELL BIOL & HISTOL LAB,RIJNSBURGERWEG 10 2333 AA LEIDEN NETHERLANDS LEIDEN UNIV,DEPT CYTOCHEM & CYTOMETRY 2333 AA LEIDEN NETHERLANDS
Titolo Testata:
Calcified tissue international
fascicolo: 2, volume: 54, anno: 1994,
pagine: 170 - 174
SICI:
0171-967X(1994)54:2<170:ODITCS>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESISTANT ACID-PHOSPHATASE; INSITU HYBRIDIZATION; MONONUCLEAR PHAGOCYTES; PARAFFIN SECTIONS; TISSUE-SECTIONS; INVITRO; RESORPTION; GENERATION; CULTURES; FIXATION;
Keywords:
COCULTURE SYSTEM; OSTEOCLAST FORMATION; IN SITU HYBRIDIZATION; MOUSE Y CHROMOSOME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
C.E. Hagenaars et al., "OSTEOCLAST DEVELOPMENT IN THE COCULTURE SYSTEM OF PERIOSTLESS METATARSAL BONES AND HEMATOPOIETIC-CELLS STUDIED BY IN-SITU HYBRIDIZATION WITH A PROBE FOR Y-CHROMOSOMES", Calcified tissue international, 54(2), 1994, pp. 170-174

Abstract

In the coculture system of periostless metatarsal bones of 17-day-oldfetal mice and osteoclast progenitors, osteoclasts will develop. Our goal in the present report was to provide further evidence that in thecoculture system of fetal metatarsal bone rudiments with hemopoietic cells, the osteoclasts developing inside the bone rudiments are exclusively derived from the cells suspended in the plasma clot and not fromendogenous precursor cells of the bone explants themselves, by using the technique of in situ hybridization with a probe for the mouse Y chromosome. Qsteoclast formation in unstripped male metatarsal rudiments, occurring after 3-4 days of culture, was compared with osteoclast formation in cocultures of female metatarsal rudiments and male bone marrow cells, occurring after 5-6 days of culture. Osteoclasts were recognized by their tartrate-resistant acid phosphatase activity. In paraffin sections of cultured male metatarsals, the mean percentage of microscopically identifiable osteoclast nuclei, in which the Y chromosome could be detected, was 43.1 +/- 4.2% (n = 12). For cocultures of femalemetatarsal bones and male bone marrow cells this mean percentage was 40.9 +/- 5.7% (n = 17). Statistical comparison by means of the two sample t-test indicated no significant difference in the percentages of osteoclast nuclei containing the Y chromosome for both groups. We concluded that the osteoclasts do derive from cocultured cells and not fromprecursor cells in the bone explant itself. Therefore, the coculture system is a reliable in vitro system for studying osteoclast formationfrom progenitor/precursor cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 15:06:13