Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CHARACTERIZATION OF APOPTOSIS IN CULTURED RAT SYMPATHETIC NEURONS AFTER NERVE GROWTH-FACTOR WITHDRAWAL
Autore:
EDWARDS SN; TOLKOVSKY AM;
Indirizzi:
UNIV CAMBRIDGE,DEPT BIOCHEM,TENNIS COURT RD CAMBRIDGE CB2 1QW ENGLAND UNIV OXFORD,DEPT HUMAN ANAT OXFORD OX1 3QX ENGLAND
Titolo Testata:
The Journal of cell biology
fascicolo: 4, volume: 124, anno: 1994,
pagine: 537 - 546
SICI:
0021-9525(1994)124:4<537:COAICR>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; PROTEIN-SYNTHESIS; PC12 CELLS; FACTOR DEPRIVATION; CYCLIC-AMP; ENDONUCLEASE; SURVIVAL; INVITRO; RNA; DIFFERENTIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
S.N. Edwards e A.M. Tolkovsky, "CHARACTERIZATION OF APOPTOSIS IN CULTURED RAT SYMPATHETIC NEURONS AFTER NERVE GROWTH-FACTOR WITHDRAWAL", The Journal of cell biology, 124(4), 1994, pp. 537-546

Abstract

Sympathetic neurons depend on nerve growth factor (NGF) for their survival both in vivo and in vitro. In culture, the neurons die after NGFwithdrawal by an autonomous cell death program but whether these neurons die by apoptosis is under debate. Using vital DNA stains and in situ nick translation, we show here that extensive chromatin condensation and DNA fragmentation occur before plasma membrane breakdown during the death of NGF-deprived rat sympathetic neurons in culture. Furthermore, kinetic analysis of chromatin condensation events within the cellpopulation is consistent with a model which postulates that after NGFdeprivation nearly all of the neurons die in this manner. Although the dying neurons display membrane blebbing, cell fragmentation into apoptotic bodies does not occur. Apoptotic events proceed rapidly at around the time neurons become committed to die, regardless of neuronal culture age. However the duration of NGF deprivation required to commit neurons to die, and the rate at which apoptosis occurs, increase with culture age. Thus, within the first week of culture, apoptosis is the predominant form of cell death in sympathetic neurons.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:40:26