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Titolo:
INFLUENCE OF GROWTH-FACTORS ON THE PLASMINOGEN-ACTIVATOR ACTIVITY OF AVIAN GRANULOSA-CELLS FROM FOLLICLES AT DIFFERENT MATURATIONAL STAGES OF PREOVULATORY DEVELOPMENT
Autore:
LAFRANCE M; CROZE F; TSANG BK;
Indirizzi:
OTTAWA CIVIC HOSP,LOEB MED RES INST,REPROD BIOL UNIT,1053 CARLING AVEOTTAWA K1Y 4E9 ON CANADA OTTAWA CIVIC HOSP,LOEB MED RES INST,REPROD BIOL UNIT OTTAWA K1Y 4E9 ON CANADA UNIV OTTAWA,DEPT OBSTET & GYNAECOL,REPROD BIOL UNIT OTTAWA K1Y 4E9 ONCANADA UNIV OTTAWA,DEPT PHYSIOL OTTAWA K1Y 4E9 ON CANADA
Titolo Testata:
Journal of molecular endocrinology
fascicolo: 3, volume: 11, anno: 1993,
pagine: 291 - 304
SICI:
0952-5041(1993)11:3<291:IOGOTP>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT GRANULOSA; TISSUE-TYPE; HORMONAL-REGULATION; DOMESTIC HEN; FACTOR-ALPHA; GONADOTROPIN REGULATION; PERIOVULATORY PERIOD; ANTI-ACTIVATOR; THECA CELLS; OVULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
M. Lafrance et al., "INFLUENCE OF GROWTH-FACTORS ON THE PLASMINOGEN-ACTIVATOR ACTIVITY OF AVIAN GRANULOSA-CELLS FROM FOLLICLES AT DIFFERENT MATURATIONAL STAGES OF PREOVULATORY DEVELOPMENT", Journal of molecular endocrinology, 11(3), 1993, pp. 291-304

Abstract

Granulosa cells from the first (F1), third (F3) and fifth and sixth (F5-6) preovulatory follicles and the small yellow follicles (SYFs; diameter 6-8 mm) were cultured for 21 h in the absence and presence of murine and human epidermal growth factors, growth fibroblast growth factor, transforming, factors alpha and beta-I (TGF alpha, TGF beta), platelet-derisred growth factor and insulin-like growth factor-I at concentrations of 0.1-100 ng/ml. Plasminogen activator (PA) activities in the cell (PAc) and in the medium (PAm) were measured by fibrinolysis andfibrin overlay methods. Basal PAc and PAm activities were highest in cell cultures from the less mature follicles (F5-6 and SYF) and decreased as the follicles matured (F3>F1). PAc activity was greater than PAm activity, irrespective of the stage of follicular development. All growth factors examined at the 100 ng/ml level were effective in increasing PAc and PAm activities in cultures of granulosa cells from F1 follicles. However, only TGF alpha was able to increase PA activities at lower concentrations. The stimulation of the PA activities of granulosa cells from F3 follicles was inconsistent. None of the growth factorssignificantly increased PA activities in granulosa cells from F5-6 follicles and SYFs, as determined by fibrinolysis. The major PAc and PAmspecies (characterized by fibrin overlay) had a molecular mass of about 35 kDa, which is characteristic of the urokinase type. Both assay methods detected a stimulatory effect of the growth factors on PA activities in the granulosa cells from F1 follicles. However, an increase in PA activities in cells from F3 and F5-6 follicles and SYFs was indicated only after fibrin overlay analysis. Tritiated thymidine was incorporated into the DNA of granulosa cells at all stages of follicular development and was enhanced by all growth factors, although TGF alpha and TGF beta were the most effective and had a ranked order of activity: F3, F5-6>F1, SYF. The present findings show that, of the growth factors examined, TGF alpha may be an effective regulator of PA activity in avian granulosa cells during follicular development, in addition to its observed mitogenic action.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 06:24:14