Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
THE APHID SALIVARY-GLAND BASAL LAMINA AS A SELECTIVE BARRIER ASSOCIATED WITH VECTOR-SPECIFIC TRANSMISSION OF BARLEY YELLOW DWARF LUTEOVIRUSES
Autore:
GILDOW FE; GRAY SM;
Indirizzi:
PENN STATE UNIV,DEPT PLANT PATHOL UNIV PK PA 16802 CORNELL UNIV,DEPT PLANT PATHOL ITHACA NY 14853
Titolo Testata:
Phytopathology
fascicolo: 12, volume: 83, anno: 1993,
pagine: 1293 - 1302
SICI:
0031-949X(1993)83:12<1293:TASBLA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACCESS PERIODS; VIRUS;
Keywords:
BASEMENT MEMBRANE; ENDOCYTOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
F.E. Gildow e S.M. Gray, "THE APHID SALIVARY-GLAND BASAL LAMINA AS A SELECTIVE BARRIER ASSOCIATED WITH VECTOR-SPECIFIC TRANSMISSION OF BARLEY YELLOW DWARF LUTEOVIRUSES", Phytopathology, 83(12), 1993, pp. 1293-1302

Abstract

The inability of virions of the MAV barley yellow dwarf luteovirus topenetrate the extracellular basal lamina surrounding the accessory salivary glands of some nonvector aphid species suggests that the basal lamina may possess a selective function that regulates vector-specificluteovirus transmission. When Sitobion avenae and Rhopalosiphum padi were fed for 2 wk on either MAV- or RPV-infected oats and then examined by electron microscopy, virions of both the transmitted MAV and the nontransmitted RPV were observed penetrating the salivary basal laminaof S. avenae. When R. padi were examined, virions of transmitted RPV consistently were observed in the salivary basal lamina; however, virions of nontransmitted MAV were not observed in the salivary basal lamina of 50 aphids examined from five experiments. When 0.5-2 ng of purified MAV was injected into individual aphids and the aphids were examined by transmission electron microscopy after a 24-h feeding on oats, virions were observed in salivary basal lamina of S. avenae and of Metopolophium dirhodum, but MAV virions were rarely observed in R. padi, R. maidis, or in Schizaphis graminum. Virions per unit basal lamina length were counted to determine relative affinity of MAV for salivary basal lamina. The average numbers of MAV virions in basal lamina of S. avenae, M. dirhodum, R. padi, R. maidis, and S. graminum were 47, 28, <1, 0, and <1 virions per 10 mu m of basal lamina length, respectively. When purified MAV virions were treated with any of three MAV-specificmonoclonal antibodies or their Fab fragments and injected into S. avenae, virions were unable to penetrate the salivary basal lamina. In addition, virions of brome mosaic virus did not penetrate the salivary basal lamina of R. padi when each aphid was injected with 30 ng of BMV. These results are consistent with the hypothesis that the accessory salivary gland basal lamina functions as a transmission barrier and that specific virus capsid-glycoprotein interactions regulate basal lamina penetration.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 02:47:38