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Titolo:
A MUTANT METALLOTHIONEIN WHICH HAS INVERSE FRAGMENT COMPOSITION EXHIBITS HIGH CADMIUM-BINDING ABILITY
Autore:
YAMAGUCHI R; KURASAKI M; KOJIMA Y;
Indirizzi:
HOKKAIDO UNIV,GRAD SCH ENVIRONM EARTH SCI,DEPT ENVIRONM MED & INFORMAT SAPPORO HOKKAIDO 060 JAPAN HOKKAIDO UNIV,GRAD SCH ENVIRONM EARTH SCI,DEPT ENVIRONM MED & INFORMAT SAPPORO HOKKAIDO 060 JAPAN
Titolo Testata:
Biochemistry and molecular biology international
fascicolo: 1, volume: 41, anno: 1997,
pagine: 49 - 56
SICI:
1039-9712(1997)41:1<49:AMMWHI>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAGNETIC-RESONANCE SPECTROSCOPY; ESCHERICHIA-COLI; METAL-BINDING; DOMAINS;
Keywords:
METALLOTHIONEIN; ALPHA-FRAGMENT; BETA-FRAGMENT; BINDING ABILITY; CD; EXPRESSION; ESCHERICHIA-COLI;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
R. Yamaguchi et al., "A MUTANT METALLOTHIONEIN WHICH HAS INVERSE FRAGMENT COMPOSITION EXHIBITS HIGH CADMIUM-BINDING ABILITY", Biochemistry and molecular biology international, 41(1), 1997, pp. 49-56

Abstract

In order to investigate the role of the alpha-fragment of metallothionein (MT) in metal-binding, two mutant MTs, beta Ala alpha Cys- and alpha Cys beta Cys-mutant MTs, expressed in Escherichia coli were analyzed for their metal-binding ability. A mutant MT where all of the cysteine residues in the beta-fragment of MT were substituted by alanine residues and another mutant MT that had the inverse fragment composition(alpha-beta, i.e., beta-alpha in wild-type MT) were designated as thebeta Ala alpha Cys and the alpha Cys beta Cys-mutant MT's, respectively. Both expressed Cd-binding mutant MTs were identified by amino acidanalyses. From their metal-binding capacities, the two mutant MTs exhibited higher Cd-binding abilities than wild-type MT. The results suggested that the alpha-fragment plays a key role in the Cd-binding of MT, and that the metal-binding tightness of MT is dependent on the metal-binding ability of a prior fragment in MT.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 08:16:45