Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ISOFORM-SPECIFIC REGULATION OF MYOCARDIAL NA,K-ATPASE ALPHA-SUBUNIT IN CONGESTIVE-HEART-FAILURE - ROLE OF NOREPINEPHRINE
Autore:
KIM CH; FAN THM; KELLY PF; HIMURA Y; DELEHANTY JM; HANG CL; LIANG CS;
Indirizzi:
UNIV ROCHESTER,MED CTR,DEPT MED,CARDIOL UNIT,BOX 679 ROCHESTER NY 14642 UNIV ROCHESTER,MED CTR,DEPT MED,CARDIOL UNIT ROCHESTER NY 14642
Titolo Testata:
Circulation
fascicolo: 1, volume: 89, anno: 1994,
pagine: 313 - 320
SICI:
0009-7322(1994)89:1<313:IROMNA>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
NA+-K+-ATPASE; CARDIAC-HYPERTROPHY; THYROID-HORMONE; GENE-EXPRESSION; CYCLIC-AMP; DOG HEART; NA+,K+-ATPASE; AFFINITY; INVIVO; BRAIN;
Keywords:
OUABAIN; HEART FAILURE, CONGESTIVE NOREPINEPHRINE; GENETICS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
C.H. Kim et al., "ISOFORM-SPECIFIC REGULATION OF MYOCARDIAL NA,K-ATPASE ALPHA-SUBUNIT IN CONGESTIVE-HEART-FAILURE - ROLE OF NOREPINEPHRINE", Circulation, 89(1), 1994, pp. 313-320

Abstract

Background Myocardial ouabain-binding sites and Na,K-ATPase activity are reduced in congestive heart failure (CHF), but the mechanisms by which CHF reduces the Na,K-ATPase remain unknown. We proposed to investigate whether the changes are accompanied by isoform-specific reductions of the Na,K-ATPase alpha-subunit proteins in CHF and whether similar changes could be produced by exogenous norepinephrine administration. Methods and Results CHF was induced in dogs by rapid ventricular pacing at a rate of 225 beats per minute for 8 weeks (protocol 1). A second group of dogs were paced at 100 beats per minute and served as controls. In protocol 2, norepinephrine was infused in normal dogs using asubcutaneous osmotic minipump for 8 weeks. The control dogs received normal saline through the pump. Animals were studied after 8 weeks of pacing or norepinephrine infusion. After the baseline hemodynamics andinterstitial norepinephrine concentration had been obtained, the hearts were removed for measuring [H-3]]ouabain-binding sites and Na,K-ATPase alpha-subunit proteins using isoform-specific monoclonal antibodies. Results Myocardial [H-3]ouabain-binding sites were reduced in dogs with CHF and chronic norepinephrine infusion. The Western blot analysis showed that adult canine hearts possess both alpha(1) and alpha(3) isoforms of the Na,K-ATPase alpha-subunit but not the alpha(2) isoform protein. CHF and NE infusion had no effect on the Na,K-ATPase alpha(1)-subunit protein but did reduce the alpha(3) isoform protein significantly. In addition, there was a significant inverse correlation betweenthe amount of myocardial alpha(3) isoform protein and interstitial norepinephrine content in the dogs. In contrast, the specific activity of the sarcolemmal marker 5'-nucleotidase did not differ among the groups of animals. Conclusions The reduction of myocardial Na,K-ATPase in CHF is limited to the alpha(3) isoform. Furthermore, because similar changes in myocardial ouabain-binding sites and Na,K-ATPase alpha(3) isoform were produced by chronic norepinephrine infusion, the decrease in the Na,K-ATPase in CHF is most likely mediated via excess sympathetic stimulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 05:23:39