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Titolo:
COEXPRESSION OF MESSENGER-RNA FOR NEUROTROPHIC FACTORS IN HUMAN NEURONS AND GLIAL-CELLS IN CULTURE
Autore:
MORETTO G; XU RY; WALKER DG; KIM SU;
Indirizzi:
UNIV BRITISH COLUMBIA,UNIV HOSP,DEPT MED,DIV NEUROL,2211 WESBROOK MALL VANCOUVER V6T 2B5 BC CANADA UNIV BRITISH COLUMBIA,UNIV HOSP,DEPT MED,DIV NEUROL VANCOUVER V6T 2B5BC CANADA UNIV BRITISH COLUMBIA,DEPT PSYCHIAT,KINSMEN LAB VANCOUVER BC CANADA
Titolo Testata:
Journal of neuropathology and experimental neurology
fascicolo: 1, volume: 53, anno: 1994,
pagine: 78 - 85
SICI:
0022-3069(1994)53:1<78:COMFNF>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; RAT-BRAIN; ALZHEIMERS-DISEASE; MOLECULAR-CLONING; GENE-EXPRESSION; MESSENGER-RNA; SEQUENCE; SYSTEM; BDNF; ACID;
Keywords:
ASTROCYTES; CULTURES; MESSENGER-RNA; NEURONS; NEUROTROPHIC FACTORS; POLYMERASE CHAIN REACTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
G. Moretto et al., "COEXPRESSION OF MESSENGER-RNA FOR NEUROTROPHIC FACTORS IN HUMAN NEURONS AND GLIAL-CELLS IN CULTURE", Journal of neuropathology and experimental neurology, 53(1), 1994, pp. 78-85

Abstract

Enriched populations of neurons and astrocytes of 93-99% purity were obtained from mixed cultures of four human fetal brains. Total cellular RNA was extracted from cell pellets and reverse transcribed into cDNA. Five mu l of cDNA were subjected to PCR amplification using primersspecific for sequences of NGF, BDNF, NT-3 and CNTF. PCR products wereseparated through 5% acrylamide gel and identified by DNA sequencing. Results showed that neurons expressed detectable levels of mRNA for NGF in all four cultures; BDNF and NT-3 mRNA was seen only in two cultures; CNTF mRNA was not detected in all four cultures. Astrocytes expressed mRNA for NGF, BDNF, and NT-3 but not for CNTF in all cultures examined. Astrocytic expression of mRNA for NGF, BDNF and NT-3 was found during the active cell proliferation as well as at a phase of mitotic quiescence. This study provides evidence that dissociated cell cultures of human neurons produce NGF, BDNF and NT-3 in early stages of theirdevelopment and that astrocytes are constitutively committed to synthesize neurotrophic factors, NGF, BDNF and NT-3. The active synthesis of selected neurotrophic factors by neurons and astrocytes is relevant in supporting migration, survival and differentiation of developing neurons in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 01:22:44