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Titolo:
DEVELOPMENT OF A METHOD FOR EMBEDDED-CULTURE OF PIG PANCREATIC ISLET-LIKE CELL CLUSTERS IN AGAROSE CONTAINING MALTOSE-CARRYING POLYSTYRENE (HEVM) AND NICOTINAMIDE
Autore:
OHGAWARA H; KOBAYASHI A; KAWAMURA M; KARIBE S; FU Q; OMORI Y; AKAIKE T;
Indirizzi:
TOKYO WOMENS MED COLL,CTR DIABET,8-1 KAWADA CHO,SHINJUKU KU TOKYO 162JAPAN TOKYO WOMENS MED COLL,MED RES INST,SHINJUKU KU TOKYO 162 JAPAN KANAGAWA ACAD SCI & TECHNOL KAWASAKI 213 JAPAN TOKYO INST TECHNOL,FAC BIOSCI & BIOTECHNOL TOKYO 152 JAPAN
Titolo Testata:
Cell transplantation
fascicolo: 1, volume: 3, anno: 1994,
pagine: 83 - 89
SICI:
0963-6897(1994)3:1<83:DOAMFE>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-FETAL PANCREAS; TISSUE-CULTURE; LANGERHANS; TRANSPLANTATION; SURVIVAL; MATRIX; DOGS; GEL;
Keywords:
EMBEDDED-CULTURE; ICCS; AGAROSE; HEVM; NICOTINAMIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
H. Ohgawara et al., "DEVELOPMENT OF A METHOD FOR EMBEDDED-CULTURE OF PIG PANCREATIC ISLET-LIKE CELL CLUSTERS IN AGAROSE CONTAINING MALTOSE-CARRYING POLYSTYRENE (HEVM) AND NICOTINAMIDE", Cell transplantation, 3(1), 1994, pp. 83-89

Abstract

A method for embedded-culture of islet-like cell clusters (ICCs) fromneonatal pig pancreas is described The procedure is based on a sequential treatment of the pancreas with a proteolytic enzyme and ethylenediaminetetraacetate (EDTA). During the exposure to EDTA-Dispase, small pieces of pancreas were gradually digested, with gentle stirring. Under- and over-digested pancreatic cell clusters were separated from the pancreatic fragments by filtration, and formed whole islet-like cell clusters in RPMI 1640 containing 11.0 MM D-glucose and 10% fetal bovineserum (FBS). The ICCs were embedded in agarose with or without a gel containing a random copolymer hydroxethylmethacrylate-vinylbenzyl maltonamide (HEVM) of hydroxethylmethacrylate-(HEMA)-vinylbenzyl maltonamide (VMA) and nicotinamide. They remained morphologically intact and a physiological response to acute stimulation with glucose was obtained when they were embedded in the agarose containing HEVM and nicotinamide. These findings suggest that ICCs in agarose containing HEVM and nicotinamide could be a useful tool for morphological, biochemical and molecular biological studies, and also as a potential source of materialfor transplantation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/08/20 alle ore 16:46:23