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Titolo:
ELECTROSTATIC AND HYDROPHOBIC INTERACTIONS ARE INVOLVED IN FACTOR-VA BINDING TO MEMBRANES CONTAINING ACIDIC PHOSPHOLIPIDS
Autore:
LECOMPTE MF; BOUIX G; MANN KG;
Indirizzi:
DEPT GLYCOCONJUGUES & BIOMEMBRANES,PHARMACOL & TOXICOL FUNDAMENTALES LAB,CNRS,118 ROUTE NARBONNE F-31062 TOULOUSE FRANCE UNIV VERMONT,DEPT BIOCHEM BURLINGTON VT 05405
Titolo Testata:
The Journal of biological chemistry
fascicolo: 3, volume: 269, anno: 1994,
pagine: 1905 - 1910
SICI:
0021-9258(1994)269:3<1905:EAHIAI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
BOVINE FACTOR-V; COAGULATION FACTOR-V; HUMAN-PLATELETS; COMPLETE CDNA; FACTOR-XA; PROTHROMBIN; VESICLES; PHOSPHATIDYLSERINE; MONOLAYERS; SUBUNITS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
M.F. Lecompte et al., "ELECTROSTATIC AND HYDROPHOBIC INTERACTIONS ARE INVOLVED IN FACTOR-VA BINDING TO MEMBRANES CONTAINING ACIDIC PHOSPHOLIPIDS", The Journal of biological chemistry, 269(3), 1994, pp. 1905-1910

Abstract

The interaction of factor Va with phospholipid monolayers was studiedusing alternating current polarography. For these studies a hanging mercury drop electrode is positioned in contact with the monolayer at the air-monolayer interface. Factor Va introduced into the aqueous subphase beneath the monolayer caused alterations in the capacitance of the circuit. When factor Va was introduced beneath monolayers containing13-25% mole fraction phosphatidylserine (PS), 87-75% mole fraction phosphatidylcholine (PC), a significant capacitance increase occurred, which is interpreted to be the result of the penetration of the factor Va molecules through the monolayer. No penetration or associated capacitance changes were observed with monolayers composed of pure PC or pure PS. Polarography experiments were also conducted with the electrodeplaced in the aqueous subphase to measure the binding of factor Va topure PS monolayers. This approach has allowed detection of factor Va (at nm concentrations) adsorption to a pure PS monolayer. Thus the membrane adsorption process can be distinguished from factor Va penetration through the monolayer. These results suggest that there are two types of interaction of the factor Va molecule with PS-containing monolayers. The interaction with the PS component of the monolayer is essential for binding the factor Va (at nm concentrations) that occurs by surface adsorption. For mixed PC/PS monolayers, PS adsorption is followedby penetration bf the protein through the membrane.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 13:13:36