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Titolo:
STATIC AND DYNAMIC STUDIES OF THE POTENTIAL-SENSITIVE MEMBRANE PROBE RH421 IN DIMYRISTOYLPHOSPHATIDYLCHOLINE VESICLES
Autore:
ZOUNI A; CLARKE RJ; VISSER AJWG; VISSER NV; HOLZWARTH JF;
Indirizzi:
MAX PLANCK GESELL,FRITZ HABER INST,FARADAYWEG 4-6 D-14195 BERLIN GERMANY MAX PLANCK GESELL,FRITZ HABER INST D-14195 BERLIN GERMANY AGR UNIV WAGENINGEN,DEPT BIOCHEM WAGENINGEN NETHERLANDS
Titolo Testata:
Biochimica et biophysica acta
fascicolo: 2, volume: 1153, anno: 1993,
pagine: 203 - 212
SICI:
0006-3002(1993)1153:2<203:SADSOT>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHARGE-SHIFT PROBES; TIME-RESOLVED FLUORESCENCE; OPTICAL PROBES; LIPID BILAYER; ELECTROCHROMIC MECHANISM; SYNAPTIC POTENTIALS; PHASE-TRANSITION; OXONOL DYES; BINDING; NEURONS;
Keywords:
POTENTIAL-SENSITIVE STYRYL DYE; STYRYL DYE; LIPID VESICLE; BINDING AFFINITY; TEMPERATURE JUMP; FLUORESCENCE LIFETIME; PHASE TRANSITION; QUANTUM YIELD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
A. Zouni et al., "STATIC AND DYNAMIC STUDIES OF THE POTENTIAL-SENSITIVE MEMBRANE PROBE RH421 IN DIMYRISTOYLPHOSPHATIDYLCHOLINE VESICLES", Biochimica et biophysica acta, 1153(2), 1993, pp. 203-212

Abstract

The dynamics of the potential-sensitive styryl dye RH421 in dimyristoylphosphatidylcholine vesicles have been investigated above and below the main phase transition temperature using iodine-laser temperature-jump relaxation spectrophotometry and time-resolved fluorescence lifetime measurements. Equilibrium fluorescence titrations have shown that the affinity of the dye for the membrane is much higher in the liquid-crystalline state than in the gel state. The interaction can be described by either a partition or a binding model and a theory is presented providing a relation between these two approaches. In the liquid-crystal line state bound dye exhibits steady-state fluorescence relaxation processes in the submicrosecond and millisecond time range following atemperature jump. Time-resolved fluorescence measurements show a variation in the fluorescence lifetime across the emission spectrum, suggesting an excited-state process occurring on the subnanosecond time scale. These processes are most likely related to dye and/or lipid reorientation following the temperature jump or excitation pulse. Temperature-dependent changes in the fluorescence excitation spectrum of bound dye suggest that the dye exists in at least two different sites within the membrane.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 09:17:15