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Titolo:
AMPLIFICATION OF THE EPIDERMAL-GROWTH-FACTOR-RECEPTOR GENE CORRELATESWITH DIFFERENT GROWTH-BEHAVIOR IN HUMAN GLIOBLASTOMA
Autore:
SCHLEGEL J; MERDES A; STUMM G; ALBERT FK; FORSTING M; HYNES N; KIESSLING M;
Indirizzi:
INST PATHOL,FRANZ JOSEF STRAUSS ALLEE 1 D-93042 REGENSBURG GERMANY UNIV HEIDELBERG,INST NEUROPATHOL HEIDELBERG GERMANY UNIV HEIDELBERG,DEPT NEUROSURG HEIDELBERG GERMANY UNIV HEIDELBERG,DEPT NEURORADIOL HEIDELBERG GERMANY FRIEDRICH MIESCHER INST BASEL SWITZERLAND
Titolo Testata:
International journal of cancer
fascicolo: 1, volume: 56, anno: 1994,
pagine: 72 - 77
SICI:
0020-7136(1994)56:1<72:AOTEGC>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
TYROSINE KINASE-ACTIVITY; HUMAN-BRAIN-TUMORS; HUMAN GLIOMAS; EGF RECEPTOR; EXPRESSION; CELLS; MULTIFORME; DOMAIN; CDNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
J. Schlegel et al., "AMPLIFICATION OF THE EPIDERMAL-GROWTH-FACTOR-RECEPTOR GENE CORRELATESWITH DIFFERENT GROWTH-BEHAVIOR IN HUMAN GLIOBLASTOMA", International journal of cancer, 56(1), 1994, pp. 72-77

Abstract

The objective of our study was to determine the frequency of EGF-receptor-gene rearrangement in relation to tumour-growth behaviour in an unselected group of glioma patients. We investigated 73 glial tumours with different grades of malignancy (17 low-grade gliomas, 14 anaplastic variants, and 42 GBM) by Southern analysis, reverse transcriptase PCR (RT-PCR) amplification of mRNA, and Western analysis. An amplification of the EGF-receptor gene was present in 19/42 GBM but in only 1 anaplastic astrocytoma. By RT-PCR, 4/19 GBM with gene amplification showed a specific amino-terminal aberrant splice mutation of 801 bp in addition to undeleted mRNA. By Western analysis, 27/42 GBM showed expression of the EGF-receptor protein. Protein levels, however, varied among individual tumours. Four GBM containing an aberrant splice mutation exhibited an immunoreactive protein of 130 kDa MW in addition to the normal EGF-receptor protein p170. All GBM patients underwent surgery followed by a standard course of radiotherapy. Neuroradiological follow-upin 31/42 GBM patients consisted of bimonthly MRI examinations. There was a statistically significant difference in the mean latency period until tumour regrowth of patients suffering from GBM with and without EGF-receptor-gene amplification (9 weeks vs. 32 weeks). Our data indicate more rapid tumour regrowth kinetics of GBM with amplified EGF receptor genes in vivo. (C) 1994 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 08:10:48