Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ANTIBODY-INDUCED APOPTOSIS IN A HUMAN LEUKEMIA-CELL LINE IS ENERGY-DEPENDENT - THERMOCHEMICAL ANALYSIS OF CELLULAR-METABOLISM
Autore:
WALLENOHMAN M; LONNBRO P; SCHON A; BORREBAECK CAK;
Indirizzi:
LUND UNIV,DEPT IMMUNOTECHNOL,POB 7031 S-22007 LUND 7 SWEDEN LUND UNIV,DEPT THERMOCHEM S-22100 LUND SWEDEN
Titolo Testata:
Cancer letters
fascicolo: 2, volume: 75, anno: 1993,
pagine: 103 - 109
SICI:
0304-3835(1993)75:2<103:AAIAHL>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCLONAL-ANTIBODY; DNA FRAGMENTATION; DEATH; TUMOR; INDUCTION; GROWTH; LYMPHOCYTES; KINETICS; LYMPHOMA; FAS;
Keywords:
MONOCLONAL ANTIBODY; APOPTOSIS; LEUKEMIA CELLS; METABOLISM; MICROCALORIMETRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
M. Wallenohman et al., "ANTIBODY-INDUCED APOPTOSIS IN A HUMAN LEUKEMIA-CELL LINE IS ENERGY-DEPENDENT - THERMOCHEMICAL ANALYSIS OF CELLULAR-METABOLISM", Cancer letters, 75(2), 1993, pp. 103-109

Abstract

The mouse monoclonal anti-BAL antibody induced apoptosis in a pre-B acute lymphocytic leukemia cell line within 2 days of incubation, afterbeing crosslinked by a secondary antibody. The antibody specifically recognized a 37 kDa membrane protein that was expressed on a wide spectrum of normal and malignant cells, but induced programmed cell death in only very few of these cells. In this study, we have followed the initial kinetics of the antibody-induced cell death in the human acute lymphocytic leukemia cell line KM-3, by microcalorimetric measurementsin conjunction with determination of the cellular proliferation rate and DNA fragmentation. An increase in metabolic activity was observed already after incubating the cells for 20 min with crosslinked anti-BAL antibody, which was several hours before significant growth inhibition and DNA fragmentation were detected. These data show for the first time that the initiation phase of antibody-induced apoptosis is an active, energy-dependent process and not merely an effect of receptor blocking.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 12:10:53