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Titolo:
STRUCTURAL ELUCIDATION OF A PEPTIDE FROM A SINGLE NEURON BY MATRIX-ASSISTED LASER DESORPTION IONIZATION EMPLOYING A TANDEM DOUBLE FOCUSING MAGNETIC-ORTHOGONAL ACCELERATION TIME-OF-FLIGHT MASS-SPECTROMETER/
Autore:
LI KW; KINGSTON R; DREISEWERD K; JIMENEZ CR; VANDERSCHORS RC; BATEMAN RH; GERAERTS WPM;
Indirizzi:
FREE UNIV AMSTERDAM,GRAD SCH NEUROSCI AMSTERDAM,NEUROSCI RES INST,DE BOELELAAN 1087 NL-1081 HV AMSTERDAM NETHERLANDS MICROMASS MANCHESTER M23 9LZ LANCS ENGLAND
Titolo Testata:
Analytical chemistry
fascicolo: 4, volume: 69, anno: 1997,
pagine: 563 - 565
SICI:
0003-2700(1997)69:4<563:SEOAPF>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
LYMNAEA-STAGNALIS; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
9
Recensione:
Indirizzi per estratti:
Citazione:
K.W. Li et al., "STRUCTURAL ELUCIDATION OF A PEPTIDE FROM A SINGLE NEURON BY MATRIX-ASSISTED LASER DESORPTION IONIZATION EMPLOYING A TANDEM DOUBLE FOCUSING MAGNETIC-ORTHOGONAL ACCELERATION TIME-OF-FLIGHT MASS-SPECTROMETER/", Analytical chemistry, 69(4), 1997, pp. 563-565

Abstract

An AutoSpec-Tof mass spectrometer, in the configuration of a matrix-assisted laser desorption/ionization source, a trisector mass spectrometer, a high-energy collision chamber, and a time-of-flight mass spectrometer, was used for direct structural characterization of a tetrapeptide from a single identifiable neuron. Peptides from the freshly dissected snail neuron were desorbed and ionized by the matrix-assisted laser desorption/ionization source. A molecular species with a protonatedmass of 429 Da was selected as the parent ion and fragmented in the high-energy collision chamber with xenon gas at 800 eV, producing daughter ions that were detected with a time-of-flight detector, The various ion series were recorded and, together, show that the parent ion hasthe primary structure of AGPWamide.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:32:55