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Titolo:
KINESIN-LIKE MOLECULES INVOLVED IN SPINDLE FORMATION
Autore:
RODIONOV VI; GELFAND VI; BORISY GG;
Indirizzi:
MOSCOW MV LOMONOSOV STATE UNIV,AN BELOZERSKY INST PHYSICOCHEM BIOL MOSCOW 119899 RUSSIA UNIV CALIF SAN FRANCISCO,DEPT BIOCHEM & BIOPHYS SAN FRANCISCO CA 94143 UNIV ILLINOIS,DEPT CELL & STRUCT BIOL URBANA IL 61801 UNIV WISCONSIN,MOLEC BIOL LAB MADISON WI 53706
Titolo Testata:
Journal of Cell Science
, volume: 106, anno: 1993,
parte:, 4
pagine: 1179 - 1188
SICI:
0021-9533(1993)106:<1179:KMIISF>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
MITOTIC APPARATUS PROTEIN; SUBCELLULAR-LOCALIZATION; CHROMOSOME MOVEMENT; CYTOPLASMIC DYNEIN; MICROTUBULE MOTOR; HEAVY-CHAIN; CENP-E; MITOSIS; CELLS; GENE;
Keywords:
KINESIN; MICROTUBULE MOTOR; MITOSIS; SPINDLE; MICROINJECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
V.I. Rodionov et al., "KINESIN-LIKE MOLECULES INVOLVED IN SPINDLE FORMATION", Journal of Cell Science, 106, 1993, pp. 1179-1188

Abstract

To study the possible involvement of kinesin-like molecules in mitosis a polyclonal antibody against the head domain of Drosophila kinesin heavy chain (HD antibody) was microinjected into PtK1 cells at the prophase-prometaphase transition. Progress of the cell through mitosis was recorded for subsequent detailed analysis. Cells injected with pre-immune IgG progressed through mitosis at rates similar to those for noninjected cells. After HD antibody injections, chromosomes failed to congress to an equatorial plane and cells failed to form a bipolar spindle. Rather, the spindle poles came together, resulting in a monopolar-like configuration with chromosomes arranged about the poles in a rosette. Sometimes the monopolar array moved to the margin of the cell in a way similar to anaphase B movement in normal cells. Antibody-injected cells progressed into the next cell cycle as evidenced by chromosomedecondensation and nuclear envelope reformation. Anti-tubulin immunofluorescence confirmed the presence of a radial monopolar array of microtubules in injected cells. HD antibody stained in a punctate pattern in interphase and the spindle region in mitotic PtK1 cells. The antibody also reacted with spindle fibers of isolated mitotic CHO spindles and with kinetochores of isolated CHO chromosomes. Immunoblotting indicated that the major component recognized by the antibody is the 120 kDa kinesin heavy chain. At higher protein loads the antibody recognizedalso a 34 kDa polypeptide in PtK1 cell extracts, a 135 kDa polypeptide in a preparation of CHO spindles and a 300 kDa polypeptide in a preparation of CHO mitotic chromosomes. We conclude that a kinesinlike molecule is important for the formation and/or maintenance of the structure of mitotic spindle.

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Documento generato il 20/10/20 alle ore 05:57:04